The inhibitory properties of middle-viscosity Chitosan on the growth of fungus Ramularia cercosporelloides were studied in vitro. The inhibitory concentration that delayed 50% of the radial growth (IC50) was 3.4 g l-1 for middle-viscosity Chitosans with molecular weights of about 133 and 187 kDa (middle molecular weight) dissolved in lactic and acetic acids, respectively. At 96 h of incubation and under the same growing conditions, inhibitions of 91.79% and 73.13% of the radial growth of the fungus were observed when 3.4 g l-1 of Chitosan was dissolved in 0.05 M acetic and 0.05 M lactic acid, respectively. The biomass production was significantly lower than that observed in the controls at 72 h. Based on these in vitro results; Chitosan could be a good alternative to control the disease caused by R. cercosporelloides on safflower.
Bibliographical noteFunding Information:
The study was funded by the Mexican Council for Science and Technology (CONACyT) through the projects 58249 and 53493 J1 at the University of Sonora and for the scholarship for postgraduate studies to E.A. Quintana-Obregón.
- Biomass production
- Carthamus tinctorius
- Ramularia cercosporelloides