Efficient disruption of escherichia coli for plasmid DNA recovery in a bead mill

Adriana Padilla-Zamudio, J. Armando Lucero-Acuña, Patricia Guerrero-Germán*, Jaime Ortega-López, Armando Tejeda-Mansir

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The release kinetics of pDNA in a bead mill was studied. Samples taken during the process were analyzed to determine total pDNA (pDNA(t)) and supercoiled pDNA (pDNA(sc)) concentration. In order to identify important variables of the process and to develop an empirical model for optimal pDNA(t) and pDNA(sc) release, a two level 23 factorial design was used with variables: mill frequency, cell concentration, and bead size. The results were analyzed by response surface methodology. The optimized conditions for pDNA(t) yield 13.26 mg/g dcw (93.41% recovery), with a mill frequency of 30 Hz, a bead size of 0.10-0.25 mm, and a cell concentration of 20 g wcw/L. However, the optimized conditions for pDNA(sc) yield 7.65 mg/g dcw (92.05% recovery), with a mill frequency of 15 Hz, a bead size of 0.10-0.25 mm, and a cell concentration of 10 g wcw/L. Cell disruption in a bead mill was proved efficient for the release of pDNA(t) and pDNA(sc) compared to the alkaline treatment. The results obtained suggest a compromise between pDNA(sc) purity and recuperation in the process development.

Original languageEnglish
Article number30
JournalApplied Sciences (Switzerland)
Volume8
Issue number1
DOIs
StatePublished - 26 Dec 2017

Bibliographical note

Publisher Copyright:
© 2017 by the authors.

Fingerprint

Dive into the research topics of 'Efficient disruption of escherichia coli for plasmid DNA recovery in a bead mill'. Together they form a unique fingerprint.

Cite this