Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering

Abraham Alejandro Leyva-Verduzco; María Mónica Castillo-Ortega; Lerma Hanaiy Chan-Chan; Erika Silva-Campa; Ramsés Galaz-Méndez; Ricardo Vera-Graziano; José Carmelo Encinas-Encinas; Teresa Del Castillo-Castro; Dora Evelia Rodríguez-Félix; Hisila del Carmen Santacruz-Ortega; Irela Santos-Sauceda

doi:10.1007/s00289-019-03057-7

Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering

Abraham Alejandro Leyva-Verduzco, María Mónica Castillo-Ortega^*, Lerma Hanaiy Chan-Chan, Erika Silva-Campa, Ramsés Galaz-Méndez, Ricardo Vera-Graziano, José Carmelo Encinas-Encinas, Teresa Del Castillo-Castro, Dora Evelia Rodríguez-Félix, Hisila del Carmen Santacruz-Ortega, Irela Santos-Sauceda

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Four tubular membranes based on PLA and gelatin with different arrangements (PLA, gelatin, PLA-gelatin Blend and PLA/gelatin Core/Shell) were prepared by the electrospinning technique, the gelatin within the materials was crosslinked with genipin and all the meshes were characterized by SEM, ATR-FTIR, TGA, DSC, XRD and mechanical tests, also a viability essay and a confocal microscopy of HUVECs in contact with the materials were carried out. A 50% decrement of cellular viability was observed in the fibers with Core/Shell structure since the first day of culture compared with the tissue culture polystyrene, due to solubilization of non-crosslinked gelatin.

Original language	English
Pages (from-to)	5985-6003
Number of pages	19
Journal	Polymer Bulletin
Volume	77
Issue number	11
DOIs	https://doi.org/10.1007/s00289-019-03057-7
State	Accepted/In press - 2019

Bibliographical note

Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

Electrospinning
Gelatin
Poly(lactic acid)
Tissue engineering

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10.1007/s00289-019-03057-7

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Leyva-Verduzco, A. A., Castillo-Ortega, M. M., Chan-Chan, L. H., Silva-Campa, E., Galaz-Méndez, R., Vera-Graziano, R., Encinas-Encinas, J. C., Del Castillo-Castro, T., Rodríguez-Félix, D. E., Santacruz-Ortega, H. D. C., & Santos-Sauceda, I. (Accepted/In press). Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering. Polymer Bulletin, 77(11), 5985-6003. https://doi.org/10.1007/s00289-019-03057-7

Leyva-Verduzco, Abraham Alejandro ; Castillo-Ortega, María Mónica ; Chan-Chan, Lerma Hanaiy ; Silva-Campa, Erika ; Galaz-Méndez, Ramsés ; Vera-Graziano, Ricardo ; Encinas-Encinas, José Carmelo ; Del Castillo-Castro, Teresa ; Rodríguez-Félix, Dora Evelia ; Santacruz-Ortega, Hisila del Carmen ; Santos-Sauceda, Irela. / Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering. In: Polymer Bulletin. 2019 ; Vol. 77, No. 11. pp. 5985-6003.

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title = "Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering",

abstract = "Four tubular membranes based on PLA and gelatin with different arrangements (PLA, gelatin, PLA-gelatin Blend and PLA/gelatin Core/Shell) were prepared by the electrospinning technique, the gelatin within the materials was crosslinked with genipin and all the meshes were characterized by SEM, ATR-FTIR, TGA, DSC, XRD and mechanical tests, also a viability essay and a confocal microscopy of HUVECs in contact with the materials were carried out. A 50% decrement of cellular viability was observed in the fibers with Core/Shell structure since the first day of culture compared with the tissue culture polystyrene, due to solubilization of non-crosslinked gelatin.",

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author = "Leyva-Verduzco, {Abraham Alejandro} and Castillo-Ortega, {Mar{\'i}a M{\'o}nica} and Chan-Chan, {Lerma Hanaiy} and Erika Silva-Campa and Rams{\'e}s Galaz-M{\'e}ndez and Ricardo Vera-Graziano and Encinas-Encinas, {Jos{\'e} Carmelo} and {Del Castillo-Castro}, Teresa and Rodr{\'i}guez-F{\'e}lix, {Dora Evelia} and Santacruz-Ortega, {Hisila del Carmen} and Irela Santos-Sauceda",

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year = "2019",

doi = "10.1007/s00289-019-03057-7",

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Leyva-Verduzco, AA, Castillo-Ortega, MM , Chan-Chan, LH , Silva-Campa, E, Galaz-Méndez, R, Vera-Graziano, R, Encinas-Encinas, JC , Del Castillo-Castro, T , Rodríguez-Félix, DE , Santacruz-Ortega, HDC & Santos-Sauceda, I 2019, 'Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering', Polymer Bulletin, vol. 77, no. 11, pp. 5985-6003. https://doi.org/10.1007/s00289-019-03057-7

Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering. / Leyva-Verduzco, Abraham Alejandro; Castillo-Ortega, María Mónica ; Chan-Chan, Lerma Hanaiy ; Silva-Campa, Erika; Galaz-Méndez, Ramsés; Vera-Graziano, Ricardo; Encinas-Encinas, José Carmelo ; Del Castillo-Castro, Teresa ; Rodríguez-Félix, Dora Evelia ; Santacruz-Ortega, Hisila del Carmen; Santos-Sauceda, Irela.

In: Polymer Bulletin, Vol. 77, No. 11, 2019, p. 5985-6003.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering

AU - Leyva-Verduzco, Abraham Alejandro

AU - Castillo-Ortega, María Mónica

AU - Chan-Chan, Lerma Hanaiy

AU - Silva-Campa, Erika

AU - Galaz-Méndez, Ramsés

AU - Vera-Graziano, Ricardo

AU - Encinas-Encinas, José Carmelo

AU - Del Castillo-Castro, Teresa

AU - Rodríguez-Félix, Dora Evelia

AU - Santacruz-Ortega, Hisila del Carmen

AU - Santos-Sauceda, Irela

PY - 2019

Y1 - 2019

N2 - Four tubular membranes based on PLA and gelatin with different arrangements (PLA, gelatin, PLA-gelatin Blend and PLA/gelatin Core/Shell) were prepared by the electrospinning technique, the gelatin within the materials was crosslinked with genipin and all the meshes were characterized by SEM, ATR-FTIR, TGA, DSC, XRD and mechanical tests, also a viability essay and a confocal microscopy of HUVECs in contact with the materials were carried out. A 50% decrement of cellular viability was observed in the fibers with Core/Shell structure since the first day of culture compared with the tissue culture polystyrene, due to solubilization of non-crosslinked gelatin.

AB - Four tubular membranes based on PLA and gelatin with different arrangements (PLA, gelatin, PLA-gelatin Blend and PLA/gelatin Core/Shell) were prepared by the electrospinning technique, the gelatin within the materials was crosslinked with genipin and all the meshes were characterized by SEM, ATR-FTIR, TGA, DSC, XRD and mechanical tests, also a viability essay and a confocal microscopy of HUVECs in contact with the materials were carried out. A 50% decrement of cellular viability was observed in the fibers with Core/Shell structure since the first day of culture compared with the tissue culture polystyrene, due to solubilization of non-crosslinked gelatin.

KW - Electrospinning

KW - Gelatin

KW - Poly(lactic acid)

KW - Tissue engineering

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U2 - 10.1007/s00289-019-03057-7

DO - 10.1007/s00289-019-03057-7

M3 - Artículo

AN - SCOPUS:85076540731

VL - 77

SP - 5985

EP - 6003

JO - Polymer Bulletin

JF - Polymer Bulletin

SN - 0170-0839

IS - 11

ER -

Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering

Abstract

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Keywords

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