Enhanced Viability and Anti-rotavirus Effect of Bifidobacterium longum and Lactobacillus plantarum in Combination With Chlorella sorokiniana in a Dairy Product

Servando Cantú-Bernal, Maribel Domínguez-Gámez, Ivana Medina-Peraza, Elizama Aros-Uzarraga, Noé Ontiveros, Lilian Flores-Mendoza, Ricardo Gomez-Flores, Patricia Tamez-Guerra, Guadalupe González-Ochoa*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Microalgae and probiotics such as Bifidobacterium and Lactobacillus genera are associated with human beneficial effects. The aim of this study was to evaluate the activity of Chlorella sorokiniana on Bifidobacterium longum and Lactobacillus plantarum viability in a dairy product (flan) and its microbial effect against rotavirus, which is one of the major diarrhea-causing pathogens worldwide. Microalge were isolated from a Mexican river and characterized by molecular tools. Their prebiotic activity was evaluated by determining Bifidobacterium longum and Lactobacillus plantarum shelf-life after incorporation in the food matrix. In addition, HT-29 cells were infected with rotavirus Wa and treated with 1 × 109 CFU/mL L. plantarum and B. longum metabolites alone or in combination with 1 × 109 cells/mL Chlorella sorokiniana; viral titers in probiotics- and/or microalgae-treated cells were evaluated for antiviral activity. Results indicated that C. sorokiniana not only significantly (p < 0.05) improved L. plantarum and B. longum viability in flan, but also increased their antiviral activity; potent anti-rotavirus effect of C. sorokiniana alone was observed. Although more studies are needed, results suggest that incorporation of this microalga into a dairy product confers enhanced viability and antiviral effects, which indicates that C. sorokiniana might be used as an ingredient to design products with additional health benefits.

Original languageEnglish
Article number875
JournalFrontiers in Microbiology
Volume11
DOIs
StatePublished - 12 May 2020

Bibliographical note

Funding Information:
The research was supported by internal research support of the Universidad de Sonora projects USO513006130 and USO513005367 to GG-O.

Funding Information:
We wish to thank Consejo Nacional de Ciencia y Tecnología (Conacyt-Mexico) for scholarship 665130 to SC-B and 1989225 to MD-G; and to Sistema Nacional de Investigadores (SNI-Conacyt- Mexico) for supporting through SNI-16614 to PT-G, SNI-9942 to RG-F, SNI-77917 to NO, SNI-54907 to LF-M, and SNI-62977 to GG-O. Funding. The research was supported by internal research support of the Universidad de Sonora projects USO513006130 and USO513005367 to GG-O.

Funding Information:
We wish to thank Consejo Nacional de Ciencia y Tecnología (Conacyt-Mexico) for scholarship 665130 to SC-B and 1989225 to MD-G; and to Sistema Nacional de Investigadores (SNI-Conacyt-Mexico) for supporting through SNI-16614 to PT-G, SNI-9942 to RG-F, SNI-77917 to NO, SNI-54907 to LF-M, and SNI-62977 to GG-O.

Publisher Copyright:
© Copyright © 2020 Cantú-Bernal, Domínguez-Gámez, Medina-Peraza, Aros-Uzarraga, Ontiveros, Flores-Mendoza, Gomez-Flores, Tamez-Guerra and González-Ochoa.

Keywords

  • flan
  • metabolites
  • microalga
  • probiotic
  • rotavirus
  • shelf-life

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