Enzymatically cross-linked arabinoxylan microspheres as oral insulin delivery system

A. L. Martínez-López, E. Carvajal-Millan*, N. Sotelo-Cruz, V. Micard, A. Rascón-Chu, Y. L. López-Franco, J. Lizardi-Mendoza, R. Canett-Romero

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Arabinoxylans (AX) microspheres with different insulin/AX mass ratio were prepared by formation of phenoxy radical issued from the ferulic acid by enzymatic oxidation (entrapped in situ of insulin). Phenolic acid content and FT-IR spectrum of unloaded and insulin-loaded AX microspheres revealed that the phenoxy radical issued from the ferulic acid by enzymatic oxidation did not interact covalently with insulin. The microspheres showed a spherical shape, smooth surface and an average diameter of particles of 320 μm. In vitro control release found that AX microspheres minimized the insulin loss in the upper GI tract, retaining high percentage (~75%) of insulin in its matrix. The stability of the secondary structure of insulin was studied by dichroism circular (CD). The CD spectra of insulin released from AX microspheres did not change according to the insulin/AX mass ratio of the microsphere. Significant hypoglycemic effects with improved insulin-relative bioavailability tested on an in vivo murine model revealed the efficacy of these enzymatically cross-linked arabinoxylans microspheres as a new oral insulin carrier.

Original languageEnglish
Pages (from-to)952-959
Number of pages8
JournalInternational Journal of Biological Macromolecules
Volume126
DOIs
StatePublished - 1 Apr 2019

Bibliographical note

Funding Information:
This research was supported by “ Fondo Institucional CONACyT - Investigación en Fronteras de la Ciencia ”, Mexico (Grant FON.INST./31/2016 to E. Carvajal-Millan). The authors are pleased to acknowledge Aurélie Putois (INRA, Montpellier, France) for phenolic acids determination and Alma C. Campa-Mada, and Karla G. Martínez-Robinson and (CIAD) for technical assistance.

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

  • Cross-linked arabinoxylans
  • Diabetes
  • Hydrogels
  • Insulin stability
  • Oral drug delivery

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