TY - JOUR
T1 - ESBL-producing Escherichia coli and Klebsiella pneumoniae from health-care institutions in Mexico
AU - Gallegos-Miranda, Viridiana
AU - Garza-Ramos, Ulises
AU - Bolado-Martínez, Enrique
AU - Navarro-Navarro, Moisés
AU - Félix-Murray, Katya Rocío
AU - Candia-Plata, Maria del Carmen
AU - Sanchez-Martinez, Guillermina
AU - Dúran-Bedolla, Josefina
AU - Silva-Sánchez, Jesús
N1 - Publisher Copyright:
© 2020 Edizioni Scientifi che per l'Informazione su Farmaci e Terapia.
PY - 2020
Y1 - 2020
N2 - We investigated the phenotypic and molecular characteristics of Extended-Spectrum-β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae clinical isolates from four health-care institutions in Hermosillo, Sonora, Mexico. ESBL-producing isolates were collected from February to August 2016. The prevalence of ESBL-producing E. coli and K. pneumoniae was 11.9 and 8.7%, respectively. High dissemination of resistance to ciprofloxacin (88%), trimethoprim/sulfamethoxazole (72%) and aminoglycosides (59%) were detected, as well as susceptibility to meropenem, amikacin and tigecycline. The ESBL found variants were CTX-M-1 (88%) and CTX-M-9 (5%). The plasmid-mediated quinolone resistance (PMQR) gene aac(6´)-Ib-cr was identified in 62% of a representative sample, whereas the qnrB and qnrS genes were detected in 49% of the isolates. PFGE analyses detected many unrelated clones among the hospital or community isolates. A constant programme of epidemiological surveillance is recommended to understand the dynamics of bacterial resistance to both cephalosporin as well as the fluoroquinolone family of antibiotics.
AB - We investigated the phenotypic and molecular characteristics of Extended-Spectrum-β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae clinical isolates from four health-care institutions in Hermosillo, Sonora, Mexico. ESBL-producing isolates were collected from February to August 2016. The prevalence of ESBL-producing E. coli and K. pneumoniae was 11.9 and 8.7%, respectively. High dissemination of resistance to ciprofloxacin (88%), trimethoprim/sulfamethoxazole (72%) and aminoglycosides (59%) were detected, as well as susceptibility to meropenem, amikacin and tigecycline. The ESBL found variants were CTX-M-1 (88%) and CTX-M-9 (5%). The plasmid-mediated quinolone resistance (PMQR) gene aac(6´)-Ib-cr was identified in 62% of a representative sample, whereas the qnrB and qnrS genes were detected in 49% of the isolates. PFGE analyses detected many unrelated clones among the hospital or community isolates. A constant programme of epidemiological surveillance is recommended to understand the dynamics of bacterial resistance to both cephalosporin as well as the fluoroquinolone family of antibiotics.
KW - PFGE
KW - PMQR
KW - aac(6´)-Ib-cr
KW - qnrB
KW - qnrS
KW - quinolone resistance
KW - β-lactamases
UR - http://www.scopus.com/inward/record.url?scp=85098558494&partnerID=8YFLogxK
U2 - 10.1080/1120009X.2020.1861815
DO - 10.1080/1120009X.2020.1861815
M3 - Artículo
C2 - 33357158
AN - SCOPUS:85098558494
SN - 1120-009X
VL - 33
SP - 122
EP - 127
JO - Journal of Chemotherapy
JF - Journal of Chemotherapy
IS - 2
ER -