Evaluation of antimicrobial susceptibility and genetic profiles (ERIC-PCR) of Enterococcus species isolated from chicken viscera

Arantxa Almada-Corral; Maritza Alejandra Cordero-Ortiz; Lizbeth Dolores Ballesteros-Herrera; Siria de Jesús  Calderón-Montoya; Enrique Bolado Martínez; Reyna Isabel Sánchez Mariñez; Alfonso García-Galaz; Iracema del Carmen  Rodríguez-Hernandez; Mario Onofre Cortez Rocha; Maritza Lizeth ÁLVAREZ-AINZA

Evaluation of antimicrobial susceptibility and genetic profiles (ERIC-PCR) of Enterococcus species isolated from chicken viscera

Arantxa Almada-Corral, Maritza Alejandra Cordero-Ortiz, Lizbeth Dolores Ballesteros-Herrera, Siria de Jesús Calderón-Montoya, Enrique Bolado Martínez, Reyna Isabel Sánchez Mariñez, Alfonso García-Galaz, Iracema del Carmen Rodríguez-Hernandez, Mario Onofre Cortez Rocha, Maritza Lizeth ÁLVAREZ-AINZA^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The genus Enterococcus can be found in water, soil, and food, and above all, they are part of the animal (including human) intestinal microbiota. Food prevalence is mainly due to their resistance to adverse environmental conditions. Seventy-three isolates from chicken viscera were confirmed to belong to the Enterococcus genus by biochemical tests and carbohydrate fermentation. Phenotypic characterization
of the species indicates that E. casseliflavus was the predominant specie. Antimicrobial resistance to amikacin (42 %), kanamycin (38 %), streptomycin (55 %) and erythromycin (33 %) in the isolates was notorious. The analysis obtained from
ERIC-PCR profiles shows a high genetic variability among the isolates. In addition, a relationship between the antimicrobial and ERIC-PCR profiles was observed among isolates. These results indicate the presence of multi-resistant Enterococcus in commercial chicken viscera with high genetic variability,
which could be a potential nosocomial bacterium or transfer this resistance to another pathogenic species causing human diseases

Translated title of the contribution	Evaluación de la susceptibilidad a antibióticos y perfiles genéticos (ERIC-PCR) de especies Enterococcus aislados de vísceras de pollo
Original language	English
Article number	DOI: 10.18633/biotecnia.v25i1.1869
Pages (from-to)	169
Number of pages	176
Journal	Biotecnia
Volume	25
Issue number	1
State	Published - 20 Jan 2023

Cite this

Almada-Corral, A., Cordero-Ortiz, M. A., Ballesteros-Herrera, L. D., Calderón-Montoya, S. D. J., Bolado Martínez, E., Sánchez Mariñez, R. I., García-Galaz, A., Rodríguez-Hernandez, I. D. C., Cortez Rocha, M. O., & ÁLVAREZ-AINZA, M. L. (2023). Evaluation of antimicrobial susceptibility and genetic profiles (ERIC-PCR) of Enterococcus species isolated from chicken viscera. Biotecnia, 25(1), 169. Article DOI: 10.18633/biotecnia.v25i1.1869.

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title = "Evaluation of antimicrobial susceptibility and genetic profiles (ERIC-PCR) of Enterococcus species isolated from chicken viscera",

abstract = "The genus Enterococcus can be found in water, soil, and food, and above all, they are part of the animal (including human) intestinal microbiota. Food prevalence is mainly due to their resistance to adverse environmental conditions. Seventy-three isolates from chicken viscera were confirmed to belong to the Enterococcus genus by biochemical tests and carbohydrate fermentation. Phenotypic characterizationof the species indicates that E. casseliflavus was the predominant specie. Antimicrobial resistance to amikacin (42 %), kanamycin (38 %), streptomycin (55 %) and erythromycin (33 %) in the isolates was notorious. The analysis obtained fromERIC-PCR profiles shows a high genetic variability among the isolates. In addition, a relationship between the antimicrobial and ERIC-PCR profiles was observed among isolates. These results indicate the presence of multi-resistant Enterococcus in commercial chicken viscera with high genetic variability,which could be a potential nosocomial bacterium or transfer this resistance to another pathogenic species causing human diseases",

keywords = "Enterococcus; antimicrobials; identification; resistance; food safety",

author = "Arantxa Almada-Corral and Cordero-Ortiz, {Maritza Alejandra} and Ballesteros-Herrera, {Lizbeth Dolores} and Calder{\'o}n-Montoya, {Siria de Jes{\'u}s} and {Bolado Mart{\'i}nez}, Enrique and {S{\'a}nchez Mari{\~n}ez}, {Reyna Isabel} and Alfonso Garc{\'i}a-Galaz and Rodr{\'i}guez-Hernandez, {Iracema del Carmen} and {Cortez Rocha}, {Mario Onofre} and {\'A}LVAREZ-AINZA, {Maritza Lizeth}",

year = "2023",

month = jan,

day = "20",

language = "Ingl{\'e}s",

volume = "25",

pages = "169",

journal = "Biotecnia",

issn = "1665-1456",

publisher = "UNIVERSIDAD DE SONORA",

number = "1",

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Almada-Corral, A, Cordero-Ortiz, MA, Ballesteros-Herrera, LD, Calderón-Montoya, SDJ, Bolado Martínez, E , Sánchez Mariñez, RI, García-Galaz, A, Rodríguez-Hernandez, IDC, Cortez Rocha, MO & ÁLVAREZ-AINZA, ML 2023, 'Evaluation of antimicrobial susceptibility and genetic profiles (ERIC-PCR) of Enterococcus species isolated from chicken viscera', Biotecnia, vol. 25, no. 1, DOI: 10.18633/biotecnia.v25i1.1869, pp. 169.

Evaluation of antimicrobial susceptibility and genetic profiles (ERIC-PCR) of Enterococcus species isolated from chicken viscera. / Almada-Corral, Arantxa; Cordero-Ortiz, Maritza Alejandra; Ballesteros-Herrera, Lizbeth Dolores et al.
In: Biotecnia, Vol. 25, No. 1, DOI: 10.18633/biotecnia.v25i1.1869, 20.01.2023, p. 169.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Evaluation of antimicrobial susceptibility and genetic profiles (ERIC-PCR) of Enterococcus species isolated from chicken viscera

AU - Almada-Corral, Arantxa

AU - Cordero-Ortiz, Maritza Alejandra

AU - Ballesteros-Herrera, Lizbeth Dolores

AU - Calderón-Montoya, Siria de Jesús

AU - Bolado Martínez, Enrique

AU - Sánchez Mariñez, Reyna Isabel

AU - García-Galaz, Alfonso

AU - Rodríguez-Hernandez, Iracema del Carmen

AU - Cortez Rocha, Mario Onofre

AU - ÁLVAREZ-AINZA, Maritza Lizeth

PY - 2023/1/20

Y1 - 2023/1/20

N2 - The genus Enterococcus can be found in water, soil, and food, and above all, they are part of the animal (including human) intestinal microbiota. Food prevalence is mainly due to their resistance to adverse environmental conditions. Seventy-three isolates from chicken viscera were confirmed to belong to the Enterococcus genus by biochemical tests and carbohydrate fermentation. Phenotypic characterizationof the species indicates that E. casseliflavus was the predominant specie. Antimicrobial resistance to amikacin (42 %), kanamycin (38 %), streptomycin (55 %) and erythromycin (33 %) in the isolates was notorious. The analysis obtained fromERIC-PCR profiles shows a high genetic variability among the isolates. In addition, a relationship between the antimicrobial and ERIC-PCR profiles was observed among isolates. These results indicate the presence of multi-resistant Enterococcus in commercial chicken viscera with high genetic variability,which could be a potential nosocomial bacterium or transfer this resistance to another pathogenic species causing human diseases

AB - The genus Enterococcus can be found in water, soil, and food, and above all, they are part of the animal (including human) intestinal microbiota. Food prevalence is mainly due to their resistance to adverse environmental conditions. Seventy-three isolates from chicken viscera were confirmed to belong to the Enterococcus genus by biochemical tests and carbohydrate fermentation. Phenotypic characterizationof the species indicates that E. casseliflavus was the predominant specie. Antimicrobial resistance to amikacin (42 %), kanamycin (38 %), streptomycin (55 %) and erythromycin (33 %) in the isolates was notorious. The analysis obtained fromERIC-PCR profiles shows a high genetic variability among the isolates. In addition, a relationship between the antimicrobial and ERIC-PCR profiles was observed among isolates. These results indicate the presence of multi-resistant Enterococcus in commercial chicken viscera with high genetic variability,which could be a potential nosocomial bacterium or transfer this resistance to another pathogenic species causing human diseases

KW - Enterococcus; antimicrobials; identification; resistance; food safety

M3 - Artículo

SN - 1665-1456

VL - 25

SP - 169

JO - Biotecnia

JF - Biotecnia

IS - 1

M1 - DOI: 10.18633/biotecnia.v25i1.1869

ER -