TY - JOUR
T1 - Evaluation of antioxidant capacity, protective effect on human erythrocytes and phenolic compound identification in two varieties of plum fruit (Spondias spp.) by UPLC-MS
AU - Hernández-Ruiz, Karen L.
AU - Ruiz-Cruz, Saul
AU - Cira-Chávez, Luis A.
AU - Gassos-Ortega, Laura E.
AU - de Jesús Ornelas-Paz, José
AU - Del-Toro-Sánchez, Carmen L.
AU - Márquez-Ríos, Enrique
AU - López-Mata, Marco A.
AU - Rodríguez-Félix, Francisco
N1 - Funding Information:
We acknowledge to Mexican Council for Science and Technology (CONACYT) for the project 270219 and post-grade scholarship granted to the first author. This research was supported by Technological Institute of Sonora through the project PROFAPI 2017-0049; 2018-0061. Also, is gratefully acknowledged to Program for Professional Development Teacher, for the Superior Type (PRODEP) by the support of second author. The APC was funded by Program for Strengthening Educational Quality (PFCE 2018).
Funding Information:
Funding: We acknowledge to Mexican Council for Science and Technology (CONACYT) for the project 270219 and post-grade scholarship granted to the first author. This research was supported by Technological Institute of Sonora through the project PROFAPI 2017-0049; 2018-0061. Also, is gratefully acknowledged to Program for Professional Development Teacher, for the Superior Type (PRODEP) by the support of second author. The APC was funded by Program for Strengthening Educational Quality (PFCE 2018).
Publisher Copyright:
© 2018 by the authors.
PY - 2018/12/4
Y1 - 2018/12/4
N2 - Plum edible part was used to obtained extracts by during a 4 h maceration process using three different solvents (ethanol, methanol and water) for the determination of total phenols and flavonoids, antioxidant capacity by (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hemolysis inhibition in human blood assays. Subsequently, phenolic compounds were identified using ultra-performance liquid chromatography (UPLC-MS). The results indicated that the ethanolic extract of plum fruit being a good source of phenolic (12–18 mg GAE/g FW) and flavonoids (2.3–2.5 mg QE/g FW) content in both varieties of plum. Also, the fruits proved a good source of antioxidants as measured by DPPH and ABTS; likewise, plum aqueous extracts showed the highest protective effect on human erythrocytes with 74.34 and 64.62% for yellow and red plum, respectively. A total of 23 bioactive compounds were identified by UPLC-MS, including gallic acid, rutin, resorcinol, chlorogenic acid, catechin, and ellagic acid, and the antioxidant capacity can be attributed to these species. The edible part of plum contains compounds of biological interest, suggesting that this fruit has antioxidant potential that can be exploited for various technologies.
AB - Plum edible part was used to obtained extracts by during a 4 h maceration process using three different solvents (ethanol, methanol and water) for the determination of total phenols and flavonoids, antioxidant capacity by (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hemolysis inhibition in human blood assays. Subsequently, phenolic compounds were identified using ultra-performance liquid chromatography (UPLC-MS). The results indicated that the ethanolic extract of plum fruit being a good source of phenolic (12–18 mg GAE/g FW) and flavonoids (2.3–2.5 mg QE/g FW) content in both varieties of plum. Also, the fruits proved a good source of antioxidants as measured by DPPH and ABTS; likewise, plum aqueous extracts showed the highest protective effect on human erythrocytes with 74.34 and 64.62% for yellow and red plum, respectively. A total of 23 bioactive compounds were identified by UPLC-MS, including gallic acid, rutin, resorcinol, chlorogenic acid, catechin, and ellagic acid, and the antioxidant capacity can be attributed to these species. The edible part of plum contains compounds of biological interest, suggesting that this fruit has antioxidant potential that can be exploited for various technologies.
KW - Antioxidant capacity
KW - Phenolic compounds
KW - Spondias spp
KW - UPLC-MS
UR - http://www.scopus.com/inward/record.url?scp=85057629227&partnerID=8YFLogxK
U2 - 10.3390/molecules23123200
DO - 10.3390/molecules23123200
M3 - Artículo
C2 - 30518166
AN - SCOPUS:85057629227
VL - 23
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 12
M1 - 3200
ER -