Extrusion Improves the Antihypertensive Potential of a Kabuli Chickpea (Cicer arietinum L.) Protein Hydrolysate

Jeanett Chávez-Ontiveros, Cuauhtémoc Reyes-Moreno, Giovanni Isaí Ramírez-Torres, Oscar Gerardo Figueroa-Salcido, Jesús Gilberto Arámburo-Gálvez, Alvaro Montoya-Rodríguez, Noé Ontiveros, Edith Oliva Cuevas-Rodríguez*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Chickpea hydrolysates could have antihypertensive potential, but there are no evaluations in vivo. Thus, the antihypertensive potential of a chickpea protein hydrolysate obtained before and after extrusion (a process that modifies protein digestibility) was evaluated. Protein precipitates were obtained from extruded and unextruded chickpea flours by isoelectric precipitation and hydrolyzed (α-amylase/pepsin/pancreatin). Chemical composition was determined (standard methods). ACE-I inhibition assays were carried out using a colorimetric test. For antihypertensive effect evaluations, spontaneously hypertensive rats (n = 8) received the treatments intragastrically (extruded or unextruded hydrolysate (1.2 g/kg), captopril (25 mg/kg), or water only). Fat, ash, and carbohydrate contents were lower in extruded chickpea flour (p < 0.05 versus unextruded). The protein content varied between protein precipitates (91.03%/78.66% unextruded/extruded (dry basis)) (p < 0.05). The hydrolysates’ IC50 values (mg/mL) were 0.2834 (unextruded)/0.3218 (extruded) (p > 0.05). All treatments lowered the blood pressure (p < 0.05 vs. water). The extruded hydrolysate showed a more potent antihypertensive effect than the unextruded one (p < 0.05), an effect similar to captopril (p > 0.05). The results suggest that protein extrusion can be used to generate protein hydrolysates with improved health benefits. The findings have implications for the design and production of functional foods that could help to prevent hypertension or serve as an adjunct in its treatment.

Original languageEnglish
Article number2562
Issue number17
StatePublished - Sep 2022

Bibliographical note

Funding Information:
This research received funding from PROFAPI-2022 Program from the Universidad Autónoma de Sinaloa, México.

Publisher Copyright:
© 2022 by the authors.


  • ACE-I
  • Kabuli
  • antihypertensive
  • chickpea
  • extrusion
  • hydrolysate
  • hypertension


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