TY - JOUR
T1 - Functionalised peptide hydrogel for the delivery of cardiac progenitor cells
AU - Castillo Diaz, Luis Alberto
AU - Saiani, Alberto
AU - Miller, Aline F
PY - 2021/2/22
Y1 - 2021/2/22
N2 - Heart failure (HF) remains one of the leading causes of death worldwide; most commonly developing after myocardial infarction (MI). Since adult cardiomyocytes characteristically do not proliferate, cells lost during MI are not replaced. As a result, the heart has a limited regenerative capacity. There is, therefore, a need to develop novel cell-based therapies to promote the regeneration of the heart after MI. The delivery and retention of cells at the injury site remains a significant challenge. In this context, we explored the potential of using an injectable, RGDSP-functionalised self-assembling peptide — FEFEFKFK — hydrogel as scaffold for the delivery and reten- tion of rat cardiac progenitor cells (CPCs) into the heart. Our results show that culturing CPCs in vitro within the hydrogel for one-week promoted their spontaneous differentiation towards adult cardiac phenotypes. Injection of the hydrogel on its own, or loaded with CPCs, into the rat after injury resulted in a significant reduction in myocardial damage and left ventricular dilation.
AB - Heart failure (HF) remains one of the leading causes of death worldwide; most commonly developing after myocardial infarction (MI). Since adult cardiomyocytes characteristically do not proliferate, cells lost during MI are not replaced. As a result, the heart has a limited regenerative capacity. There is, therefore, a need to develop novel cell-based therapies to promote the regeneration of the heart after MI. The delivery and retention of cells at the injury site remains a significant challenge. In this context, we explored the potential of using an injectable, RGDSP-functionalised self-assembling peptide — FEFEFKFK — hydrogel as scaffold for the delivery and reten- tion of rat cardiac progenitor cells (CPCs) into the heart. Our results show that culturing CPCs in vitro within the hydrogel for one-week promoted their spontaneous differentiation towards adult cardiac phenotypes. Injection of the hydrogel on its own, or loaded with CPCs, into the rat after injury resulted in a significant reduction in myocardial damage and left ventricular dilation.
UR - http://dx.doi.org/10.1016/j.msec.2020.111539
U2 - 10.1016/j.msec.2020.111539
DO - 10.1016/j.msec.2020.111539
M3 - Artículo
C2 - 33321610
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -