TY - JOUR
T1 - Functionalization of chitosan by a free radical reaction
T2 - Characterization, antioxidant and antibacterial potential
AU - Moreno-Vásquez, María Jesús
AU - Buitimea-Valenzuela, Emma Lucía
AU - Plascencia-Jatomea, Maribel
AU - Encinas-Encinas, José Carmelo
AU - Rodríguez-Félix, Francisco
AU - Sánchez-Valdes, Saúl
AU - Rosas-Burgos, Ema Carina
AU - Ocaño-Higuera, Víctor Manuel
AU - Graciano-Verdugo, Abril Zoraida
N1 - Publisher Copyright:
© 2016 Elsevier Ltd
PY - 2017/1/2
Y1 - 2017/1/2
N2 - Chitosan was functionalized with epigallocatechin gallate (EGCG) by a free radical-induced grafting procedure, which was carried out by a redox pair (ascorbic acid/hydrogen peroxide) as the radical initiator. The successful preparation of EGCG grafted-chitosan was verified by spectroscopic (UV, FTIR and XPS) and thermal (DSC and TGA) analyses. The degree of grafting of phenolic compounds onto the chitosan was determined by the Folin-Ciocalteu procedure. Additionally, the biological activities (antioxidant and antibacterial) of pure EGCG, blank chitosan and EGCG grafted-chitosan were evaluated. The spectroscopic and thermal results indicate chitosan functionalization with EGCG; the EGCG content was 25.8 mg/g of EGCG grafted-chitosan. The antibacterial activity of the EGCG grafted-chitosan was increased compared to pure EGCG or blank chitosan against S. aureus and Pseudomonas sp. (p < 0.05). Additionally, EGCG grafted-chitosan showed higher antioxidant activity than blank chitosan. These results indicate that EGCG grafted-chitosan might be useful in active food packaging.
AB - Chitosan was functionalized with epigallocatechin gallate (EGCG) by a free radical-induced grafting procedure, which was carried out by a redox pair (ascorbic acid/hydrogen peroxide) as the radical initiator. The successful preparation of EGCG grafted-chitosan was verified by spectroscopic (UV, FTIR and XPS) and thermal (DSC and TGA) analyses. The degree of grafting of phenolic compounds onto the chitosan was determined by the Folin-Ciocalteu procedure. Additionally, the biological activities (antioxidant and antibacterial) of pure EGCG, blank chitosan and EGCG grafted-chitosan were evaluated. The spectroscopic and thermal results indicate chitosan functionalization with EGCG; the EGCG content was 25.8 mg/g of EGCG grafted-chitosan. The antibacterial activity of the EGCG grafted-chitosan was increased compared to pure EGCG or blank chitosan against S. aureus and Pseudomonas sp. (p < 0.05). Additionally, EGCG grafted-chitosan showed higher antioxidant activity than blank chitosan. These results indicate that EGCG grafted-chitosan might be useful in active food packaging.
KW - Antibacterial
KW - Antioxidant
KW - Epigallocatechin gallate
KW - Grafting
KW - Modified chitosan
UR - http://www.scopus.com/inward/record.url?scp=84983593636&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2016.08.056
DO - 10.1016/j.carbpol.2016.08.056
M3 - Artículo
SN - 0144-8617
VL - 557
SP - 117
EP - 127
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
IS - 1-2
ER -