Functionalized Liposomes Fabrication and Characterization with Eryth-rocruorin for a Potential Application as a Red Blood Cells Substitute

Nadia García-Flores, César Rodríguez-Beas, Ramón Alfonso Iñiguez-Palomares, José Manuel Galván-Moroyoqui, Juan Manuel Martínez-Soto, Ricardo López-Esparza, Ericka Rodríguez-León*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: Erythrocruorin is considered for a potential application as a Red Blood Cells Sub-stitute. Background: Nanostructured materials such as liposomes and polymeric materials have offered new alternatives that allow the design of systems as potential substitutes for red blood cells. Objective: Functionalized liposomes fabrication and characterization. Methods: The liposomes manufactured with egg yolk lecithin prepared by the extrusion method were functionalized with EfEc and stabilized with polyethylene glycol (PEG) by an absorption method. Results: The nanoplatform has a hydrodynamic diameter of 452.4 ± 24.56 nm; this size provides the ability to avoid problems such as system extravasation, also presents stability (ζ-potential =-30. 06 ± 0.42 mV) and SEM micrographs show a semi-spherical morphology. Also, EfEc showed a peak of fluorescence emission at 449 nm, which allowed us to observe the protein's presence on the surface of the liposomes employing CLSM. Conclusion: In this work, we report the fabrication and characterization of liposomes with EfEc and PEG on their surface. The nanoplatform exhibits physical-chemical characteristics that confer a potential application as a substitute for red blood cells.

Original languageEnglish
Pages (from-to)910-920
Number of pages11
JournalCurrent Nanoscience
Volume17
Issue number6
DOIs
StatePublished - Nov 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2021 Bentham Science Publishers.

Keywords

  • Eisenia fetida
  • Erythrocruorin
  • Lecithin
  • Liposomes
  • Polyethylene glycol
  • Red blood cell substitutes

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