TY - JOUR
T1 - Genetic characterization of plasmid-mediated fluoroquinolone efflux pump QepA among ESBL-producing Escherichia coli isolates in Mexico
AU - Bacterial Resistance Consortium
AU - Duran-Bedolla, Josefina
AU - Garza-Ramos, Ulises
AU - Silva-Sánchez, Jesús
AU - Lozano, Luis
AU - Reyna-Flores, Fernando
AU - Sánchez-Pérez, Alejandro
AU - Barrios-Camacho, Humberto
AU - Uribe-Salas, Felipe J.
AU - Navarro, Moisés
AU - Cornejo-Juárez, Patricia
AU - Novoa-Farías, Octavio
AU - Solórzano, Fortino
AU - Cruz, Sofía
N1 - Publisher Copyright:
© 2023, The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.
PY - 2023/12
Y1 - 2023/12
N2 - Antimicrobial resistance is a major global public health problem, with fluoroquinolone-resistant strains of Escherichia coli posing a significant threat. This study examines the genetic characterization of ESBL-producing E. coli isolates in Mexican hospitals, which are resistant to both cephalosporins and fluoroquinolones. A total of 23 ESBL-producing E. coli isolates were found to be positive for the qepA gene, which confers resistance to fluoroquinolones. These isolates exhibited drug resistance phenotypes and belonged to specific sequence types and phylogenetic groups. The genetic context of the qepA gene was identified in a novel genetic context flanked by IS26 sequences. Mating experiments showed the co-transfer of qepA1 and chrA determinants alongside bla CTX-M-15 genes, emphasizing the potential for these genetic structures to spread among Enterobacterales. The emergence of multidrug-resistant Gram-negative bacteria carrying these resistance genes is a significant clinical concern for public healthcare systems.
AB - Antimicrobial resistance is a major global public health problem, with fluoroquinolone-resistant strains of Escherichia coli posing a significant threat. This study examines the genetic characterization of ESBL-producing E. coli isolates in Mexican hospitals, which are resistant to both cephalosporins and fluoroquinolones. A total of 23 ESBL-producing E. coli isolates were found to be positive for the qepA gene, which confers resistance to fluoroquinolones. These isolates exhibited drug resistance phenotypes and belonged to specific sequence types and phylogenetic groups. The genetic context of the qepA gene was identified in a novel genetic context flanked by IS26 sequences. Mating experiments showed the co-transfer of qepA1 and chrA determinants alongside bla CTX-M-15 genes, emphasizing the potential for these genetic structures to spread among Enterobacterales. The emergence of multidrug-resistant Gram-negative bacteria carrying these resistance genes is a significant clinical concern for public healthcare systems.
KW - ESBL
KW - Efflux pump qepA
KW - Escherichia coli
KW - Fluoroquinolone
UR - http://www.scopus.com/inward/record.url?scp=85171198283&partnerID=8YFLogxK
U2 - 10.1007/s42770-023-01115-x
DO - 10.1007/s42770-023-01115-x
M3 - Artículo
C2 - 37702924
AN - SCOPUS:85171198283
SN - 1517-8382
VL - 54
SP - 2791
EP - 2797
JO - Brazilian Journal of Microbiology
JF - Brazilian Journal of Microbiology
IS - 4
ER -