Genotypic and phenotypic diversity of Alicyclobacillus acidocaldarius isolates

L. Félix-Valenzuela; I. Guardiola-Avila; A. Burgara-Estrella; M. Ibarra-Zavala; V. Mata-Haro

doi:10.1111/lam.12464

Genotypic and phenotypic diversity of Alicyclobacillus acidocaldarius isolates

L. Félix-Valenzuela, I. Guardiola-Avila, A. Burgara-Estrella, M. Ibarra-Zavala, V. Mata-Haro^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The fruit juice industry recognizes Alicyclobacillus as a major quality control target micro-organism. In this study, we analysed 19 bacterial isolates to identify Alicyclobacillus species by polymerase chain reaction (PCR) and sequencing analyses. Phenotypic and genomic diversity among isolates were investigated by API 50CHB system and ERIC-PCR (enterobacterial repetitive intergenic consensus-PCR) respectively. All bacterial isolates were identified as Alicyclobacillus acidocaldarius, and almost all showed identical DNA sequences according to their 16S rRNA (rDNA) gene partial sequences. Only few carbohydrates were fermented by A. acidocaldarius isolates, and there was little variability in the biochemical profile. Genotypic fingerprinting of the A. acidocaldarius isolates showed high diversity, and clusters by ERIC-PCR were distinct to those obtained from the 16S rRNA gene phylogenetic tree. There was no correlation between phenotypic and genotypic variability in the A. acidocaldarius isolates analysed in this study.

Original language	English
Pages (from-to)	367-373
Number of pages	7
Journal	Letters in Applied Microbiology
Volume	61
Issue number	4
DOIs	https://doi.org/10.1111/lam.12464
State	Published - 1 Oct 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015 The Society for Applied Microbiology.

Keywords

Alicyclobacillus acidocaldarius
ERIC-PCR
Genotyping
Microbial contamination
Quality control
Spoilage

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10.1111/lam.12464

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title = "Genotypic and phenotypic diversity of Alicyclobacillus acidocaldarius isolates",

abstract = "The fruit juice industry recognizes Alicyclobacillus as a major quality control target micro-organism. In this study, we analysed 19 bacterial isolates to identify Alicyclobacillus species by polymerase chain reaction (PCR) and sequencing analyses. Phenotypic and genomic diversity among isolates were investigated by API 50CHB system and ERIC-PCR (enterobacterial repetitive intergenic consensus-PCR) respectively. All bacterial isolates were identified as Alicyclobacillus acidocaldarius, and almost all showed identical DNA sequences according to their 16S rRNA (rDNA) gene partial sequences. Only few carbohydrates were fermented by A. acidocaldarius isolates, and there was little variability in the biochemical profile. Genotypic fingerprinting of the A. acidocaldarius isolates showed high diversity, and clusters by ERIC-PCR were distinct to those obtained from the 16S rRNA gene phylogenetic tree. There was no correlation between phenotypic and genotypic variability in the A. acidocaldarius isolates analysed in this study.",

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T1 - Genotypic and phenotypic diversity of Alicyclobacillus acidocaldarius isolates

AU - Félix-Valenzuela, L.

AU - Guardiola-Avila, I.

AU - Burgara-Estrella, A.

AU - Ibarra-Zavala, M.

AU - Mata-Haro, V.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - The fruit juice industry recognizes Alicyclobacillus as a major quality control target micro-organism. In this study, we analysed 19 bacterial isolates to identify Alicyclobacillus species by polymerase chain reaction (PCR) and sequencing analyses. Phenotypic and genomic diversity among isolates were investigated by API 50CHB system and ERIC-PCR (enterobacterial repetitive intergenic consensus-PCR) respectively. All bacterial isolates were identified as Alicyclobacillus acidocaldarius, and almost all showed identical DNA sequences according to their 16S rRNA (rDNA) gene partial sequences. Only few carbohydrates were fermented by A. acidocaldarius isolates, and there was little variability in the biochemical profile. Genotypic fingerprinting of the A. acidocaldarius isolates showed high diversity, and clusters by ERIC-PCR were distinct to those obtained from the 16S rRNA gene phylogenetic tree. There was no correlation between phenotypic and genotypic variability in the A. acidocaldarius isolates analysed in this study.

AB - The fruit juice industry recognizes Alicyclobacillus as a major quality control target micro-organism. In this study, we analysed 19 bacterial isolates to identify Alicyclobacillus species by polymerase chain reaction (PCR) and sequencing analyses. Phenotypic and genomic diversity among isolates were investigated by API 50CHB system and ERIC-PCR (enterobacterial repetitive intergenic consensus-PCR) respectively. All bacterial isolates were identified as Alicyclobacillus acidocaldarius, and almost all showed identical DNA sequences according to their 16S rRNA (rDNA) gene partial sequences. Only few carbohydrates were fermented by A. acidocaldarius isolates, and there was little variability in the biochemical profile. Genotypic fingerprinting of the A. acidocaldarius isolates showed high diversity, and clusters by ERIC-PCR were distinct to those obtained from the 16S rRNA gene phylogenetic tree. There was no correlation between phenotypic and genotypic variability in the A. acidocaldarius isolates analysed in this study.

KW - Alicyclobacillus acidocaldarius

KW - ERIC-PCR

KW - Genotyping

KW - Microbial contamination

KW - Quality control

KW - Spoilage

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DO - 10.1111/lam.12464

M3 - Artículo

C2 - 26174059

SN - 0266-8254

VL - 61

SP - 367

EP - 373

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 4

ER -

Genotypic and phenotypic diversity of Alicyclobacillus acidocaldarius isolates

Abstract

Bibliographical note

Keywords

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