Glycoxidated ferritin induces the release of microparticles positive for Toll-like receptors derived from peripheral blood CD14+ cells

Luis López Soto, Jose Galván Moroyoqui, Juan Martínez Soto, Martín Almada Balderrama, Ashley Rosales Ruíz, Gerardo Álvarez Hernández, Alma Camacho Villa, Enrique Bolado Martínez, Jesús Soto Guzmán, Maria Candia Plata

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Both increased serum ferritin levels and Toll-like receptor (TLR) activation show independent association with the inflammatory processes. During inflammation, cell activation and apoptosis are accompanied by the release of membrane-derived microparticles (MPs), which are considered to be mediators of intercellular communication as they induce specific responses in target cells. The aim of this study was to determine whether glycated and glycoxidated ferritin induce in vitro release TLR microparticles from CD14+ peripheral blood mononuclear cells. Peripheral blood mononuclear cells were stimulated with glycated, glycoxidated and native ferritin. The release of microparticles from CD14+ cells, the presence of TLR2+ and TLR4+ on the microparticles surface and the presence of interleukins-6 and -8 (IL-6 and IL-8) inside the microparticles after stimulation were determined by flow cytometry. The role of nuclear factor κB (NF-κB) was evaluated by pretreatment of the cells with the Bay 11-7085 inhibitor. Glycated and glycoxidated ferritin induced the release of microparticles from CD14+ cells, the majority of which expressed TLR2+ and TLR4+ on their surface and contained IL-6 and IL-8. These effects were dependent on NF-κB activation. Our findings show that glycated and glycoxidated ferritin might be involved in the release of microparticles and stimulation of inflammatory responses.https://doi.org/10.2298/ABS160614106LReceived: June 14, 2016; Revised: August 20, 2016; Accepted: August 22, 2016; Published online: October 31, 2016How to cite: López-Soto LF, Galván-Moroyoqui JM, Martínez-Soto JM, Almada-Balderrama M, Rosales-Ruiz AP, Álvarez-Hernández G, Camacho Villa AY, Bolado Martínez E, Soto-Guzmán JA, Candia-Plata MDC. Glycoxidated ferritin induces the release of microparticles positive for toll-like receptors derived from peripheral blood CD14+ cells. Arch Biol Sci. 2017;69(3):383-90.
Original languageEnglish
Pages (from-to)383-390
Number of pages8
JournalArchives of Biological Sciences
Volume69
Issue number3
StatePublished - 2017

Keywords

  • microparticles
  • Toll-like receptor (TLR)
  • ferritin
  • glycation
  • inflammation

Cite this

@article{30fede9477594d2aac79efe46c48f036,
title = "Glycoxidated ferritin induces the release of microparticles positive for Toll-like receptors derived from peripheral blood CD14+ cells",
abstract = "Both increased serum ferritin levels and Toll-like receptor (TLR) activation show independent association with the inflammatory processes. During inflammation, cell activation and apoptosis are accompanied by the release of membrane-derived microparticles (MPs), which are considered to be mediators of intercellular communication as they induce specific responses in target cells. The aim of this study was to determine whether glycated and glycoxidated ferritin induce in vitro release TLR microparticles from CD14+ peripheral blood mononuclear cells. Peripheral blood mononuclear cells were stimulated with glycated, glycoxidated and native ferritin. The release of microparticles from CD14+ cells, the presence of TLR2+ and TLR4+ on the microparticles surface and the presence of interleukins-6 and -8 (IL-6 and IL-8) inside the microparticles after stimulation were determined by flow cytometry. The role of nuclear factor κB (NF-κB) was evaluated by pretreatment of the cells with the Bay 11-7085 inhibitor. Glycated and glycoxidated ferritin induced the release of microparticles from CD14+ cells, the majority of which expressed TLR2+ and TLR4+ on their surface and contained IL-6 and IL-8. These effects were dependent on NF-κB activation. Our findings show that glycated and glycoxidated ferritin might be involved in the release of microparticles and stimulation of inflammatory responses.https://doi.org/10.2298/ABS160614106LReceived: June 14, 2016; Revised: August 20, 2016; Accepted: August 22, 2016; Published online: October 31, 2016How to cite: L{\'o}pez-Soto LF, Galv{\'a}n-Moroyoqui JM, Mart{\'i}nez-Soto JM, Almada-Balderrama M, Rosales-Ruiz AP, {\'A}lvarez-Hern{\'a}ndez G, Camacho Villa AY, Bolado Mart{\'i}nez E, Soto-Guzm{\'a}n JA, Candia-Plata MDC. Glycoxidated ferritin induces the release of microparticles positive for toll-like receptors derived from peripheral blood CD14+ cells. Arch Biol Sci. 2017;69(3):383-90.",
keywords = "microparticles, Toll-like receptor (TLR), ferritin, glycation, inflammation",
author = "Soto, {Luis L{\'o}pez} and Moroyoqui, {Jose Galv{\'a}n} and Soto, {Juan Mart{\'i}nez} and Balderrama, {Mart{\'i}n Almada} and Ru{\'i}z, {Ashley Rosales} and Hern{\'a}ndez, {Gerardo {\'A}lvarez} and Villa, {Alma Camacho} and Mart{\'i}nez, {Enrique Bolado} and Guzm{\'a}n, {Jes{\'u}s Soto} and Plata, {Maria Candia}",
year = "2017",
language = "Ingl{\'e}s",
volume = "69",
pages = "383--390",
journal = "Archives of Biological Sciences",
issn = "0354-4664",
publisher = "Institut za Bioloska Istrazivanja",
number = "3",

}

Glycoxidated ferritin induces the release of microparticles positive for Toll-like receptors derived from peripheral blood CD14+ cells. / Soto, Luis López; Moroyoqui, Jose Galván; Soto, Juan Martínez; Balderrama, Martín Almada; Ruíz, Ashley Rosales; Hernández, Gerardo Álvarez; Villa, Alma Camacho; Martínez, Enrique Bolado; Guzmán, Jesús Soto; Plata, Maria Candia.

In: Archives of Biological Sciences, Vol. 69, No. 3, 2017, p. 383-390.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Glycoxidated ferritin induces the release of microparticles positive for Toll-like receptors derived from peripheral blood CD14+ cells

AU - Soto, Luis López

AU - Moroyoqui, Jose Galván

AU - Soto, Juan Martínez

AU - Balderrama, Martín Almada

AU - Ruíz, Ashley Rosales

AU - Hernández, Gerardo Álvarez

AU - Villa, Alma Camacho

AU - Martínez, Enrique Bolado

AU - Guzmán, Jesús Soto

AU - Plata, Maria Candia

PY - 2017

Y1 - 2017

N2 - Both increased serum ferritin levels and Toll-like receptor (TLR) activation show independent association with the inflammatory processes. During inflammation, cell activation and apoptosis are accompanied by the release of membrane-derived microparticles (MPs), which are considered to be mediators of intercellular communication as they induce specific responses in target cells. The aim of this study was to determine whether glycated and glycoxidated ferritin induce in vitro release TLR microparticles from CD14+ peripheral blood mononuclear cells. Peripheral blood mononuclear cells were stimulated with glycated, glycoxidated and native ferritin. The release of microparticles from CD14+ cells, the presence of TLR2+ and TLR4+ on the microparticles surface and the presence of interleukins-6 and -8 (IL-6 and IL-8) inside the microparticles after stimulation were determined by flow cytometry. The role of nuclear factor κB (NF-κB) was evaluated by pretreatment of the cells with the Bay 11-7085 inhibitor. Glycated and glycoxidated ferritin induced the release of microparticles from CD14+ cells, the majority of which expressed TLR2+ and TLR4+ on their surface and contained IL-6 and IL-8. These effects were dependent on NF-κB activation. Our findings show that glycated and glycoxidated ferritin might be involved in the release of microparticles and stimulation of inflammatory responses.https://doi.org/10.2298/ABS160614106LReceived: June 14, 2016; Revised: August 20, 2016; Accepted: August 22, 2016; Published online: October 31, 2016How to cite: López-Soto LF, Galván-Moroyoqui JM, Martínez-Soto JM, Almada-Balderrama M, Rosales-Ruiz AP, Álvarez-Hernández G, Camacho Villa AY, Bolado Martínez E, Soto-Guzmán JA, Candia-Plata MDC. Glycoxidated ferritin induces the release of microparticles positive for toll-like receptors derived from peripheral blood CD14+ cells. Arch Biol Sci. 2017;69(3):383-90.

AB - Both increased serum ferritin levels and Toll-like receptor (TLR) activation show independent association with the inflammatory processes. During inflammation, cell activation and apoptosis are accompanied by the release of membrane-derived microparticles (MPs), which are considered to be mediators of intercellular communication as they induce specific responses in target cells. The aim of this study was to determine whether glycated and glycoxidated ferritin induce in vitro release TLR microparticles from CD14+ peripheral blood mononuclear cells. Peripheral blood mononuclear cells were stimulated with glycated, glycoxidated and native ferritin. The release of microparticles from CD14+ cells, the presence of TLR2+ and TLR4+ on the microparticles surface and the presence of interleukins-6 and -8 (IL-6 and IL-8) inside the microparticles after stimulation were determined by flow cytometry. The role of nuclear factor κB (NF-κB) was evaluated by pretreatment of the cells with the Bay 11-7085 inhibitor. Glycated and glycoxidated ferritin induced the release of microparticles from CD14+ cells, the majority of which expressed TLR2+ and TLR4+ on their surface and contained IL-6 and IL-8. These effects were dependent on NF-κB activation. Our findings show that glycated and glycoxidated ferritin might be involved in the release of microparticles and stimulation of inflammatory responses.https://doi.org/10.2298/ABS160614106LReceived: June 14, 2016; Revised: August 20, 2016; Accepted: August 22, 2016; Published online: October 31, 2016How to cite: López-Soto LF, Galván-Moroyoqui JM, Martínez-Soto JM, Almada-Balderrama M, Rosales-Ruiz AP, Álvarez-Hernández G, Camacho Villa AY, Bolado Martínez E, Soto-Guzmán JA, Candia-Plata MDC. Glycoxidated ferritin induces the release of microparticles positive for toll-like receptors derived from peripheral blood CD14+ cells. Arch Biol Sci. 2017;69(3):383-90.

KW - microparticles

KW - Toll-like receptor (TLR)

KW - ferritin

KW - glycation

KW - inflammation

M3 - Artículo

VL - 69

SP - 383

EP - 390

JO - Archives of Biological Sciences

JF - Archives of Biological Sciences

SN - 0354-4664

IS - 3

ER -