Heterogeneity of active sites in recombinant betaine aldehyde dehydrogenase is modulated by potassium

César Muñoz-Bacasehua, Jesús A. Rosas-Rodríguez, Aldo A. Arvizu-Flores, Aurora Stephens-Camacho, José G. Soñanez-Organis, Ciria G. Figueroa-Soto, Elisa M. Valenzuela-Soto*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Betaine aldehyde dehydrogenase (BADH EC 1.2.1.8) catalyzes the irreversible oxidation of betaine aldehyde to glycine betaine using NAD+ as a coenzyme. Porcine kidney BADH (pkBADH) follows a bi-bi ordered mechanism in which NAD+ binds to the enzyme before the aldehyde. Previous studies showed that NAD+ induces complex and unusual conformational changes on pkBADH and that potassium is required to maintain its quaternary structure. The aim of this work was to analyze the structural changes in pkBADH caused by NAD+ binding and the role played by potassium in those changes. The pkBADH cDNA was cloned and overexpressed in Escherichia coli, and the protein was purified by affinity chromatography using a chitin matrix. The pkBADH/NAD+ interaction was analyzed by circular dichroism (CD) and by isothermal titration calorimetry (ITC) by titrating the enzyme with NAD+. The cDNA has an open reading frame of 1485 bp and encodes a protein of 494 amino acids, with a predicted molecular mass of 53.9 kDa. CD data showed that the binding of NAD+ to the enzyme caused changes in its secondary structure, whereas the presence of K+ helps maintain its α-helix content. K+ increased the thermal stability of the pkBADH-NAD+ complex by 5.3°C. ITC data showed that NAD+ binding occurs with different association constants for each active site between 37.5 and 8.6 μM. All the results support previous data in which the enzyme incubation with NAD+ provoked changes in reactivity, which is an indication of slow conformational rearrangements of the active site.

Original languageEnglish
Article numbere2869
JournalJournal of Molecular Recognition
Volume33
Issue number10
DOIs
StateAccepted/In press - 2020

Bibliographical note

Funding Information:
C.M.B. gratefully acknowledges a scholarship from CONACyT for graduate studies. Authors are grateful to Dr. G. Garza‐Ramos for providing facilities and constant support to carry out CD spectra.

Publisher Copyright:
© 2020 John Wiley & Sons Ltd

Keywords

  • active sites heterogeneity
  • betaine aldehyde dehydrogenase
  • conformational rearrangements
  • glycine betaine
  • NAD binding

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