Hydrogel with silver nanoparticles synthesized by Mimosa tenuiflora for second-degree burns treatment

Aaron Martínez-Higuera, César Rodríguez-Beas, Jesús Mauro Adolfo Villalobos-Noriega, Abraham Arizmendi-Grijalva, Carlos Ochoa-Sánchez, Eduardo Larios-Rodríguez, Juan Manuel Martínez-Soto, Ericka Rodríguez-León, Cristina Ibarra-Zazueta, Roberto Mora-Monroy, Hugo Alejandro Borbón-Nuñez, Alfonso García-Galaz, María del Carmen Candia-Plata, Luis Fernando López-Soto, Ramón Iñiguez-Palomares*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

In this work we use Mimosa tenuiflora (MtE) extracts as reducing agents to synthesize silver nanoparticles (AgMt NPs) which were characterized by DPPH and Total Polyphenols Assays, UV–visible, X-ray diffractometer (XRD), high-resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS) and Thermogravimetric analysis (TGA). AgMt NPs possess average sizes of 21 nm and fcc crystalline structure, it was also confirmed that the MtE is present in the AgMt NPs even after the cleaning protocol applied. Subsequently, carbopol hydrogels were made and the MtE and the synthesized AgMt NPs were dispersed in different gels (MtE-G and AgMt NPs-G, respectively) at 100 µg/g concentration. The gels were characterized by UV–Vis, IR, and rheology. Antimicrobial tests were performed using Staphylococcus aureus and Escherichia coli. Burn wound healing was evaluated in a second-degree burn injury on a Wistar rats model for 14 days and additional skin biopsies were examined with histopathological analysis. Gel with commercial silver nanoparticles (Ag NPs) was prepared and employed as a control on the biological assays. Hydrogel system containing silver nanoparticles synthesized with Mimosa tenuiflora (AgMt NPs-G) is a promising therapeutic strategy for burn wound healing, this due to bactericidal and anti-inflammatory effects, which promotes a more effective recovery (in percentage terms) by damaged area.

Original languageEnglish
Article number11312
JournalScientific Reports
Volume11
Issue number1
DOIs
StatePublished - Dec 2021
Externally publishedYes

Bibliographical note

Funding Information:
AMH would like to thank Conacyt for Post-Doctoral Fellowship (2019-000019-01NACV-00449), Support number 740180. JMAVN, AAG, and COS would like to thank Conacyt for fellowship number 748633, 784014, and 723576, respectively. All authors wish to thank the graduate program in nanotechnology at the University of Sonora for DLS, Zeta-sizer, and SEM characterizations support. The use of the Molecular Sciences Research Center Shared Facilities, University of Puerto Rico is greatly appreciated. The X-ray micro diffractometer (Rigaku, XtaLAB SuperNova) was obtained with the support of the National Science Foundation (NSF) under the Major Research Instrumentation Program (CHE-1626103). The authors would like to thank Dra. Judith Tánori by the support in equipment for rheological experiments.

Publisher Copyright:
© 2021, The Author(s).

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