In vitro activity to promote plant growth and biological control of rhizobacteria isolated from bermuda grass ruderal

María Eugenia Rentería Martínez, Andrés Ochoa Meza, Juan Manuel Guzmán-Ortiz, Miguel ángel Barrera Silva, Ernesto Fernandez Herrera, Sergio Francisco Moreno Salazar

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Currently, the use of plant growth promoting bacteria is a viable alternative to the indiscriminate use of agrochemicals. In order to obtain bacteria with the capacity to promote plant growth, in this work twelve strains with nitrogen fixation activity were isolated from the rhizosphere of bermuda grass (Cyonodon dactylon) ruderal. Based on the speed and intensity of the color change in the qualitative nitrogen fixation test, two isolates were selected for molecular identification and characterization. The amplification, sequencing and analysis of 16S rRNA ribosomal gene fragments allowed to identify Pseudomonas monteilii (Pm0710) and Chryseobacterium massiliae ca (Cm0711). Both isolates showed phosphate solubilization activity, production of indole-3-acetic acid, gibberellic acid and siderophores, alkaline phosphatase and ACC deaminase, as well as inhibition against: Fusarium brachygibbosum, F. falciforme, F. oxysporum and Ceratobasidium sp. Although Cm0711 was significantly higher than Pm0710 in most of the properties analyzed, the two bacteria have potential to be used as bio-inoculants; however, before validation must be done in vivo.
Original languageSpanish (Mexico)
Pages (from-to)311-324
Number of pages15
JournalRevista Mexicana de Ciencias Agrícolas
Volume10
Issue number2
StatePublished - 15 Mar 2019

Cite this

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title = "In vitro activity to promote plant growth and biological control of rhizobacteria isolated from bermuda grass ruderal",
abstract = "Currently, the use of plant growth promoting bacteria is a viable alternative to the indiscriminate use of agrochemicals. In order to obtain bacteria with the capacity to promote plant growth, in this work twelve strains with nitrogen fixation activity were isolated from the rhizosphere of bermuda grass (Cyonodon dactylon) ruderal. Based on the speed and intensity of the color change in the qualitative nitrogen fixation test, two isolates were selected for molecular identification and characterization. The amplification, sequencing and analysis of 16S rRNA ribosomal gene fragments allowed to identify Pseudomonas monteilii (Pm0710) and Chryseobacterium massiliae ca (Cm0711). Both isolates showed phosphate solubilization activity, production of indole-3-acetic acid, gibberellic acid and siderophores, alkaline phosphatase and ACC deaminase, as well as inhibition against: Fusarium brachygibbosum, F. falciforme, F. oxysporum and Ceratobasidium sp. Although Cm0711 was significantly higher than Pm0710 in most of the properties analyzed, the two bacteria have potential to be used as bio-inoculants; however, before validation must be done in vivo.",
keywords = "Ceratobasidium, Chryseobacterium massiliae, Fusarium, Pseudomonas monteilii, PGPR",
author = "{Renter{\'i}a Mart{\'i}nez}, {Mar{\'i}a Eugenia} and {Ochoa Meza}, Andr{\'e}s and Guzm{\'a}n-Ortiz, {Juan Manuel} and {Barrera Silva}, {Miguel {\'a}ngel} and {Fernandez Herrera}, Ernesto and {Moreno Salazar}, {Sergio Francisco}",
year = "2019",
month = "3",
day = "15",
language = "Espa{\~n}ol (M{\'e}xico)",
volume = "10",
pages = "311--324",
journal = "Revista Mexicana de Ciencias Agr{\'i}colas",
issn = "2007-9230",
number = "2",

}

In vitro activity to promote plant growth and biological control of rhizobacteria isolated from bermuda grass ruderal. / Rentería Martínez, María Eugenia; Ochoa Meza, Andrés; Guzmán-Ortiz, Juan Manuel; Barrera Silva, Miguel ángel; Fernandez Herrera, Ernesto; Moreno Salazar, Sergio Francisco.

In: Revista Mexicana de Ciencias Agrícolas, Vol. 10, No. 2, 15.03.2019, p. 311-324.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - In vitro activity to promote plant growth and biological control of rhizobacteria isolated from bermuda grass ruderal

AU - Rentería Martínez, María Eugenia

AU - Ochoa Meza, Andrés

AU - Guzmán-Ortiz, Juan Manuel

AU - Barrera Silva, Miguel ángel

AU - Fernandez Herrera, Ernesto

AU - Moreno Salazar, Sergio Francisco

PY - 2019/3/15

Y1 - 2019/3/15

N2 - Currently, the use of plant growth promoting bacteria is a viable alternative to the indiscriminate use of agrochemicals. In order to obtain bacteria with the capacity to promote plant growth, in this work twelve strains with nitrogen fixation activity were isolated from the rhizosphere of bermuda grass (Cyonodon dactylon) ruderal. Based on the speed and intensity of the color change in the qualitative nitrogen fixation test, two isolates were selected for molecular identification and characterization. The amplification, sequencing and analysis of 16S rRNA ribosomal gene fragments allowed to identify Pseudomonas monteilii (Pm0710) and Chryseobacterium massiliae ca (Cm0711). Both isolates showed phosphate solubilization activity, production of indole-3-acetic acid, gibberellic acid and siderophores, alkaline phosphatase and ACC deaminase, as well as inhibition against: Fusarium brachygibbosum, F. falciforme, F. oxysporum and Ceratobasidium sp. Although Cm0711 was significantly higher than Pm0710 in most of the properties analyzed, the two bacteria have potential to be used as bio-inoculants; however, before validation must be done in vivo.

AB - Currently, the use of plant growth promoting bacteria is a viable alternative to the indiscriminate use of agrochemicals. In order to obtain bacteria with the capacity to promote plant growth, in this work twelve strains with nitrogen fixation activity were isolated from the rhizosphere of bermuda grass (Cyonodon dactylon) ruderal. Based on the speed and intensity of the color change in the qualitative nitrogen fixation test, two isolates were selected for molecular identification and characterization. The amplification, sequencing and analysis of 16S rRNA ribosomal gene fragments allowed to identify Pseudomonas monteilii (Pm0710) and Chryseobacterium massiliae ca (Cm0711). Both isolates showed phosphate solubilization activity, production of indole-3-acetic acid, gibberellic acid and siderophores, alkaline phosphatase and ACC deaminase, as well as inhibition against: Fusarium brachygibbosum, F. falciforme, F. oxysporum and Ceratobasidium sp. Although Cm0711 was significantly higher than Pm0710 in most of the properties analyzed, the two bacteria have potential to be used as bio-inoculants; however, before validation must be done in vivo.

KW - Ceratobasidium

KW - Chryseobacterium massiliae

KW - Fusarium

KW - Pseudomonas monteilii

KW - PGPR

M3 - Artículo

VL - 10

SP - 311

EP - 324

JO - Revista Mexicana de Ciencias Agrícolas

JF - Revista Mexicana de Ciencias Agrícolas

SN - 2007-9230

IS - 2

ER -