In vitro studies of the effects of aflatoxin B1 and fumonisin B1 on trypsin-like and collagenase-like activity from the hepatopancreas of white shrimp (Litopenaeus vannamei)

Armando Burgos-Hernández; Sergio I. Farias; Wilfrido Torres-Arreola; Josafat M. Ezquerra-Brauer

doi:10.1016/j.aquaculture.2005.05.024

In vitro studies of the effects of aflatoxin B₁ and fumonisin B₁ on trypsin-like and collagenase-like activity from the hepatopancreas of white shrimp (Litopenaeus vannamei)

Armando Burgos-Hernández, Sergio I. Farias, Wilfrido Torres-Arreola, Josafat M. Ezquerra-Brauer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The in vitro effects of aflatoxin B₁ (AFB₁) and fumonisin B₁ (FB₁) on the trypsin-like and collagenase-like activity from hepatopancreas (HP) of cultured white shrimp (Litopenaeus vannamei) have been studied. The presence of trypsin-like and collagenase-like enzymes in the shrimp HP was confirmed by inhibition and electrophoresis analysis. Both enzymes were strongly inhibited by phenylmethylsulfonyl fluoride (PMSF) which suggests that they belong to serine proteases. Collagenase-like protease digested native porcine type I collagen. Trypsin-like activity was inhibited by AFB₁ and FB₁. The collagenase-like activity was stimulated by FB₁. Lineweaver-Burk reciprocal plots indicated uncompetitive inhibition of trypsin-like enzyme by AFB₁. Competitive inhibition in collagenase-like enzyme, and increased K_m and V_max values of collagenase-like enzyme were observed when exposed to FB₁. These findings may suggest that AFB₁ and FB₁ toxicity to shrimp might be due to digestive deficiency and consequential histological changes in its hepatopancreas, as a result of alterations of trypsin and collagenase activity due to the presence of mycotoxins in the shrimp diet.

Original language	English
Pages (from-to)	399-410
Number of pages	12
Journal	Aquaculture
Volume	250
Issue number	1-2
DOIs	https://doi.org/10.1016/j.aquaculture.2005.05.024
State	Published - 14 Nov 2005

Bibliographical note

Funding Information:
The authors thank Jose Luis Cárdenas López for his suggestions during the preparation of the manuscript. This study was supported by CONACYT 31600-B grant given to author Josafat Marina Ezquerra Brauer.

Keywords

Hepatopancreas
Litopenaeus vannamei
Mycotoxins
Protease

Access to Document

10.1016/j.aquaculture.2005.05.024

Cite this

@article{16cff0ed8ee04e139a4ccd9cd9892eae,

title = "In vitro studies of the effects of aflatoxin B1 and fumonisin B1 on trypsin-like and collagenase-like activity from the hepatopancreas of white shrimp (Litopenaeus vannamei)",

abstract = "The in vitro effects of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) on the trypsin-like and collagenase-like activity from hepatopancreas (HP) of cultured white shrimp (Litopenaeus vannamei) have been studied. The presence of trypsin-like and collagenase-like enzymes in the shrimp HP was confirmed by inhibition and electrophoresis analysis. Both enzymes were strongly inhibited by phenylmethylsulfonyl fluoride (PMSF) which suggests that they belong to serine proteases. Collagenase-like protease digested native porcine type I collagen. Trypsin-like activity was inhibited by AFB1 and FB1. The collagenase-like activity was stimulated by FB1. Lineweaver-Burk reciprocal plots indicated uncompetitive inhibition of trypsin-like enzyme by AFB1. Competitive inhibition in collagenase-like enzyme, and increased Km and Vmax values of collagenase-like enzyme were observed when exposed to FB1. These findings may suggest that AFB1 and FB1 toxicity to shrimp might be due to digestive deficiency and consequential histological changes in its hepatopancreas, as a result of alterations of trypsin and collagenase activity due to the presence of mycotoxins in the shrimp diet.",

keywords = "Hepatopancreas, Litopenaeus vannamei, Mycotoxins, Protease",

author = "Armando Burgos-Hern{\'a}ndez and Farias, {Sergio I.} and Wilfrido Torres-Arreola and Ezquerra-Brauer, {Josafat M.}",

note = "Funding Information: The authors thank Jose Luis C{\'a}rdenas L{\'o}pez for his suggestions during the preparation of the manuscript. This study was supported by CONACYT 31600-B grant given to author Josafat Marina Ezquerra Brauer.",

year = "2005",

month = nov,

day = "14",

doi = "10.1016/j.aquaculture.2005.05.024",

language = "Ingl{\'e}s",

volume = "250",

pages = "399--410",

journal = "Aquaculture",

issn = "0044-8486",

publisher = "Elsevier",

number = "1-2",

}

In vitro studies of the effects of aflatoxin B₁ and fumonisin B₁ on trypsin-like and collagenase-like activity from the hepatopancreas of white shrimp (Litopenaeus vannamei). / Burgos-Hernández, Armando; Farias, Sergio I.; Torres-Arreola, Wilfrido et al.
In: Aquaculture, Vol. 250, No. 1-2, 14.11.2005, p. 399-410.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - In vitro studies of the effects of aflatoxin B1 and fumonisin B1 on trypsin-like and collagenase-like activity from the hepatopancreas of white shrimp (Litopenaeus vannamei)

AU - Burgos-Hernández, Armando

AU - Farias, Sergio I.

AU - Torres-Arreola, Wilfrido

AU - Ezquerra-Brauer, Josafat M.

N1 - Funding Information: The authors thank Jose Luis Cárdenas López for his suggestions during the preparation of the manuscript. This study was supported by CONACYT 31600-B grant given to author Josafat Marina Ezquerra Brauer.

PY - 2005/11/14

Y1 - 2005/11/14

N2 - The in vitro effects of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) on the trypsin-like and collagenase-like activity from hepatopancreas (HP) of cultured white shrimp (Litopenaeus vannamei) have been studied. The presence of trypsin-like and collagenase-like enzymes in the shrimp HP was confirmed by inhibition and electrophoresis analysis. Both enzymes were strongly inhibited by phenylmethylsulfonyl fluoride (PMSF) which suggests that they belong to serine proteases. Collagenase-like protease digested native porcine type I collagen. Trypsin-like activity was inhibited by AFB1 and FB1. The collagenase-like activity was stimulated by FB1. Lineweaver-Burk reciprocal plots indicated uncompetitive inhibition of trypsin-like enzyme by AFB1. Competitive inhibition in collagenase-like enzyme, and increased Km and Vmax values of collagenase-like enzyme were observed when exposed to FB1. These findings may suggest that AFB1 and FB1 toxicity to shrimp might be due to digestive deficiency and consequential histological changes in its hepatopancreas, as a result of alterations of trypsin and collagenase activity due to the presence of mycotoxins in the shrimp diet.

AB - The in vitro effects of aflatoxin B1 (AFB1) and fumonisin B1 (FB1) on the trypsin-like and collagenase-like activity from hepatopancreas (HP) of cultured white shrimp (Litopenaeus vannamei) have been studied. The presence of trypsin-like and collagenase-like enzymes in the shrimp HP was confirmed by inhibition and electrophoresis analysis. Both enzymes were strongly inhibited by phenylmethylsulfonyl fluoride (PMSF) which suggests that they belong to serine proteases. Collagenase-like protease digested native porcine type I collagen. Trypsin-like activity was inhibited by AFB1 and FB1. The collagenase-like activity was stimulated by FB1. Lineweaver-Burk reciprocal plots indicated uncompetitive inhibition of trypsin-like enzyme by AFB1. Competitive inhibition in collagenase-like enzyme, and increased Km and Vmax values of collagenase-like enzyme were observed when exposed to FB1. These findings may suggest that AFB1 and FB1 toxicity to shrimp might be due to digestive deficiency and consequential histological changes in its hepatopancreas, as a result of alterations of trypsin and collagenase activity due to the presence of mycotoxins in the shrimp diet.

KW - Hepatopancreas

KW - Litopenaeus vannamei

KW - Mycotoxins

KW - Protease

UR - http://www.scopus.com/inward/record.url?scp=27344443080&partnerID=8YFLogxK

U2 - 10.1016/j.aquaculture.2005.05.024

DO - 10.1016/j.aquaculture.2005.05.024

M3 - Artículo

SN - 0044-8486

VL - 250

SP - 399

EP - 410

JO - Aquaculture

JF - Aquaculture

IS - 1-2

ER -