Intracellular Redox Status and Cell Death Induced by H2O2 in a Human Retinal Epithelial Cell Line (ARPE-19)

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Abstract

Hydrogen peroxide is a normal by-product of cellular metabolism that in higher concentrations can cause
oxidative stress. Reactive oxygen species impair the physiological functions of retinal pigment epithelial (RPE) cells, which
are known as one major cause of ocular pathologies. Most studies investigating the influence of H2O2 on cells in culture but
H2O2 concentrations are not sustained in culture medium. Continuous generation using glucose oxidase (GOx) system allows
application of relevant low H2O2 concentrations over physiologically relevant times periods (up to 24 h). Recent findings
suggest that bolus and GOx treatments can lead to different cellular response, thus warranting a quantitative comparison
between the two approaches. When added as a pulse H2O2 is rapidly depleted. Continuous generation of H2O2 produces
different behavior in function of GOx activities. Cytotoxicity analyses show that cells can tolerate short exposure to high H2O2
doses delivered as a pulse but are susceptible to lower continuous doses. Application of hydrogen peroxide causes a
concentration-dependent decrease in the intracellular glutathione (GSH) content that was accompanied by a matching decrease
in the glutathione peroxide activity and reducing power (FRAP).
Original languageEnglish
Article number3
Pages (from-to)1-21
Number of pages21
JournalAmerican Journal of BioScience
Volume3
Issue number3
StatePublished - 12 May 2015

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