ISOLATION AND IDENTIFICATION OF A NEW ANTIPROLIFERATIVE INDOLOCARBAZOLE ALKALOID DERIVATIVE EXTRACTED FROM FARMED SHRIMP (Litopenaeus vannamei) MUSCLE

Joel Said García-Romo, Martín Samuel Hernández-Zazueta, Alma Carolina Galvez-Iriqui, Maribel Plascencia-Jatomea, María Guadalupe Burboa-Zazueta, Edgar Sandoval-Petris, Rosario Maribel Robles-Sánchez, Josué Elías Júarez-Onofre, Javier Hernández-Martínez, Hisila Del Carmen Santacruz-Ortega, Carmen María López-Saiz, Armando Burgos-Hernández*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Farmed shrimp Litopenaeus vannamei, one of the most consumed seafood in the planet, is a source of antiproliferative extracts still to be fully characterized. This study the isolation and identification of these antiproliferative compounds. From a chloroform-soluble extract from shrimp muscle, hexaneand methanol-soluble fractions were obtained and tested for antiproliferative activity (MTT), a bioassay that guided the fractionation and isolation of bioactive fractions using open column chromatography. MeOH-soluble fraction resulted bioactive and was subjected to further fractionation from which one subfraction outstand for being highly active against prostate cancer cell line. Antiproliferative effects were evaluated using colorimetric assays and cell morphology observations. Further chromatographic procedures resulted in sub-fractions from which one was effective in causing DNA damage and F-actin polymerization, which suggests cellular collapse and apoptosis. According to the structural chemical characterization carried out, dioctyl phthalate, eicosapentaenoic acid, and an indolocarbazole alkaloid type of compound were identified. This last compound, which resulted majorly responsible for the bioactivity, was not found reported in the available databases. Pure EPA control was used to compare it with the subfraction, observing greater activity in the subfraction than when EPA was used, suggesting that another compound different from EPA is providing the highest activity; but, more investigation is needed for a full chemical and structural characterization.

Original languageEnglish
Article numbere2173
JournalJournal of Microbiology, Biotechnology and Food Sciences
Volume11
Issue number5
DOIs
StatePublished - Apr 2022

Bibliographical note

Funding Information:
Authors acknowledge the Consejo Nacional de Ciencia y Tecnolog?a (CONACYT) M?xico for granting graduate scholarship and financial support to project 241133.

Funding Information:
Acknowledgments: Authors acknowledge the Consejo Nacional de Ciencia y Tecnología (CONACYT) México for granting graduate scholarship and financial support to project 241133.

Publisher Copyright:
© 2022

Keywords

  • Litopenaeus vannamei
  • bioactive compounds
  • marine source

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