La sucralosa promueve la polarización a macrófagos proinflamatorios M1

Translated title of the contribution: Sucralose promotes pro-inflammatory M1 macrophage polarizatio

N. Aurora Stephens-Camacho, Jesús A. Rosas Rodríguez, Anna P. Islas-Zamorano, Javier A. Magaña-Gómez, Lilian K. Flores-Mendoza*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Sucralose is a non-nutritive sweetener widely consumed worldwide; it is considered a safe additive because it is eliminated quickly. Recently its bioaccumulation in adipose tissue was evidenced, where macrophages, cells of the immune system involved in developing low-grade systemic inflammation, are found. To date, there is a paucity of information regarding whether sweeteners potentiate inflammatory processes by altering the function of cells present in tissue and/or contri-bute to the development of metabolic pathologies. We evaluate the effect of sucralose on the viability of differentiated macrophages of the monocytic cell line THP-1, by trypan blue and MTT assays, respectively, as well as its effect on M1/ M2 by PCR according to the expression of IRF4, IRF5, STAT1, STAT6, expression profile of IL6, IL-12, TNF-α, TGF-β, IL-10 and SOCS3 by qPCR, and the quantification of the chemokine IP-10 by ELISE. The results indicated that sucralose has no cytotoxic effects but decreases the number of metabolically active viable cells determined by MTT of macrophages in a concentration-dependent manner. Sucralose increased the concentration of the chemokine IP-10 and the gene expression of the transcription factors IRF5 and decreased the expression of IRF4 and STAT 6 gene expression, favoring polarization towards M1 populations. The bioaccumulation of sucralose in adipose tissue, and its interaction with macrophages, could induce its polarization to M1.

Translated title of the contributionSucralose promotes pro-inflammatory M1 macrophage polarizatio
Original languageSpanish
Pages (from-to)578-587
Number of pages10
JournalRevista Chilena de Nutricion
Issue number5
StatePublished - 1 Oct 2022

Bibliographical note

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© 2022, Sociedad Chilena de Nutricion Bromatologia y Toxilogica. All rights reserved.


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