TY - JOUR
T1 - Lysyl oxidase from jumbo squid (Dosidicus gigas) muscle
T2 - Detection and partial purification
AU - Torres-Arreola, Wilfrido
AU - Ezquerra-Brauer, Josafat M.
AU - Figueroa-Soto, Ciria G.
AU - Valenzuela-Soto, Elisa M.
AU - Garcia-Sanchez, Guillermina
AU - Marquez-Rios, Enrique
AU - Pacheco-Aguilar, Ramón
PY - 2011/8
Y1 - 2011/8
N2 - Lysyl oxidase (LOX) was detected and partially purified from jumbo squid (Dosidicus gigas) muscle, for the first time. A procedure for the purification of LOX from jumbo squid muscle which consisted of urea extraction, Sephadex G-75 and anion exchange chromatography was developed. Activity of partially purified LOX was 390-fold higher than the original extract. Two protein fractions with 32 and 24kDa were detected by SDS-PAGE. The enzyme was strongly inhibited by β-aminopropionitrile fumarate, a specific LOX inhibitor. LOX was purified with 3.8% yield, showing a specific activity of 0.078IUmg-1 protein. This knowledge will help understand the behaviour of jumbo squid protein during cool storage or manufacture.
AB - Lysyl oxidase (LOX) was detected and partially purified from jumbo squid (Dosidicus gigas) muscle, for the first time. A procedure for the purification of LOX from jumbo squid muscle which consisted of urea extraction, Sephadex G-75 and anion exchange chromatography was developed. Activity of partially purified LOX was 390-fold higher than the original extract. Two protein fractions with 32 and 24kDa were detected by SDS-PAGE. The enzyme was strongly inhibited by β-aminopropionitrile fumarate, a specific LOX inhibitor. LOX was purified with 3.8% yield, showing a specific activity of 0.078IUmg-1 protein. This knowledge will help understand the behaviour of jumbo squid protein during cool storage or manufacture.
KW - Jumbo squid
KW - Lysyl oxidase
KW - Purification
KW - Tentacles
UR - http://www.scopus.com/inward/record.url?scp=79959981011&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2621.2011.02676.x
DO - 10.1111/j.1365-2621.2011.02676.x
M3 - Artículo
SN - 0950-5423
VL - 46
SP - 1711
EP - 1715
JO - International Journal of Food Science and Technology
JF - International Journal of Food Science and Technology
IS - 8
ER -