Lysyl oxidase from jumbo squid (Dosidicus gigas) muscle: Detection and partial purification

Wilfrido Torres-Arreola, Josafat M. Ezquerra-Brauer, Ciria G. Figueroa-Soto, Elisa M. Valenzuela-Soto, Guillermina Garcia-Sanchez, Enrique Marquez-Rios, Ramón Pacheco-Aguilar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Lysyl oxidase (LOX) was detected and partially purified from jumbo squid (Dosidicus gigas) muscle, for the first time. A procedure for the purification of LOX from jumbo squid muscle which consisted of urea extraction, Sephadex G-75 and anion exchange chromatography was developed. Activity of partially purified LOX was 390-fold higher than the original extract. Two protein fractions with 32 and 24kDa were detected by SDS-PAGE. The enzyme was strongly inhibited by β-aminopropionitrile fumarate, a specific LOX inhibitor. LOX was purified with 3.8% yield, showing a specific activity of 0.078IUmg-1 protein. This knowledge will help understand the behaviour of jumbo squid protein during cool storage or manufacture.

Original languageEnglish
Pages (from-to)1711-1715
Number of pages5
JournalInternational Journal of Food Science and Technology
Issue number8
StatePublished - Aug 2011


  • Jumbo squid
  • Lysyl oxidase
  • Purification
  • Tentacles


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