Molecular detection of equine infectious anemia virus in clinically normal, seronegative horses in an endemic area of Mexico

César I. Romo-Sáenz, Patricia Tamez-Guerra, Aymee Olivas-Holguin, Yareellys Ramos-Zayas, Nelson Obregón-Macías, Guadalupe González-Ochoa, Francisco J. Zavala-Díaz de la Serna, Cristina Rodríguez-Padilla, Reyes Tamez-Guerra, Ricardo Gomez-Flores*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Equine infectious anemia (EIA) is a highly infectious disease in members of the Equidae family, caused by equine infectious anemia virus (EIAV). The disease severity ranges from subclinical to acute or chronic, and causes significant economic losses in the equine industry worldwide. Serologic tests for detection of EIAV infection have some concerns given the prolonged seroconversion time. Therefore, molecular methods are needed to improve surveillance programs for this disease. We attempted detection of EIAV in 6 clinical and 42 non-clinical horses in Nuevo Leon State, Mexico, using the agar gel immunodiffusion (AGID) test for antibody detection, and nested and hemi-nested PCR for detection of proviral DNA. We found that 6 of 6, 5 of 6, and 6 of 6 clinical horses were positive by AGID, nested PCR, and hemi-nested PCR, respectively, whereas 0 of 42, 1 of 42, and 9 of 42 non-clinical horses were positive by these tests, respectively. BLAST analysis of the 203-bp 5′-LTR/tat segment of PCR product revealed 83–93% identity with EIAV isolates in GenBank and reference strains from other countries. By phylogenetic analysis, our Mexican samples were grouped in a different clade than other sequences reported worldwide, indicating that the LRT/tat region represents an important target for the detection of non-clinical horses.

Original languageEnglish
Pages (from-to)758-761
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume33
Issue number4
DOIs
StatePublished - Jul 2021

Bibliographical note

Funding Information:
This study was supported by a grant from Programa de Apoyo a la Investigación Científica y Tecnológica-UANL 2019 (grant CN-933) to Ricardo Gomez-Flores and by internal research support of Universidad de Sonora grant USO513006999 to Guadalupe González-Ochoa.

Funding Information:
We thank Laboratorio de Inmunología y Virología of Facultad de Ciencias Biológicas at Universidad Autónoma de Nuevo León (FCB-UANL) for supporting the development of this study. This study was supported by a grant from Programa de Apoyo a la Investigación Científica y Tecnológica-UANL 2019 (grant CN-933) to Ricardo Gomez-Flores and by internal research support of Universidad de Sonora grant USO513006999 to Guadalupe González-Ochoa.

Publisher Copyright:
© 2021 The Author(s).

Keywords

  • asymptomatic infection
  • equine infectious anemia virus
  • horses
  • viral disease

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