TY - JOUR
T1 - Molecular interaction of β-carotene with sweet potato starch
T2 - A bleaching-restitution assay
AU - Escobar-Puentes, Alberto A.
AU - Reyes-López, Simón Yobanny
AU - Jesús Ruíz Baltazar, Álvaro de
AU - López-Teros, Verónica
AU - Wall-Medrano, Abraham
N1 - Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/6
Y1 - 2022/6
N2 - This work aimed to investigate the most plausible molecular interactions between β-carotene (βCat) and orange-fleshed sweet potato (OFSP) starch, by using a bleaching-restitution strategy. OFSP's raw flour (RF) was partially (TF) and extensively (starch) bleached with a graded polarity solvent system. The in vitro interaction (saturated restitution) of pure βCat to starch (βCat + S) at 2.5, 5, 10 g.100 g−1 was tracked by FE-SEM, ATR-FTIR, XRD and changes in starch digestibility [slow (SDS), rapid (RDS), and resistant (RS)] were documented. βCat + S interaction occurs mostly by H+ bonding (inter and intra-helical) and CH–π stacking (alkene chains - glucose rings), altering starch's molecular order and crystallinity, RS: SDS ratio and increasing intra/inter type-V inclusion and non-inclusion complexes, in a dose-dependent manner. Data suggests that βCat + S is governed by non-covalent forces promoting starch's resistance to digestion, probably influencing the bioaccessibility and bioavailability of both βCat and glucose monomers.
AB - This work aimed to investigate the most plausible molecular interactions between β-carotene (βCat) and orange-fleshed sweet potato (OFSP) starch, by using a bleaching-restitution strategy. OFSP's raw flour (RF) was partially (TF) and extensively (starch) bleached with a graded polarity solvent system. The in vitro interaction (saturated restitution) of pure βCat to starch (βCat + S) at 2.5, 5, 10 g.100 g−1 was tracked by FE-SEM, ATR-FTIR, XRD and changes in starch digestibility [slow (SDS), rapid (RDS), and resistant (RS)] were documented. βCat + S interaction occurs mostly by H+ bonding (inter and intra-helical) and CH–π stacking (alkene chains - glucose rings), altering starch's molecular order and crystallinity, RS: SDS ratio and increasing intra/inter type-V inclusion and non-inclusion complexes, in a dose-dependent manner. Data suggests that βCat + S is governed by non-covalent forces promoting starch's resistance to digestion, probably influencing the bioaccessibility and bioavailability of both βCat and glucose monomers.
KW - Digestibility
KW - Orange-fleshed sweet potato
KW - Resistant starch
KW - Starch
KW - β-carotene
UR - http://www.scopus.com/inward/record.url?scp=85123258046&partnerID=8YFLogxK
U2 - 10.1016/j.foodhyd.2022.107522
DO - 10.1016/j.foodhyd.2022.107522
M3 - Artículo
AN - SCOPUS:85123258046
SN - 0268-005X
VL - 127
JO - Food Hydrocolloids
JF - Food Hydrocolloids
M1 - 107522
ER -