Mucosal immune response of spotted sand bass Paralabrax maculatofasciatus (Steindachner, 1868) orally immunised with an extracellular lectin of Aeromonas veronii

M. L. Merino-Contreras, M. A. Guzman-Murillo, E. Ruiz-Bustos, M. J. Romero, M. A. Cadena-Roa, F. Ascencio*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

To assess the immunogenic and immunoprotective role of the extracellular lectin from Aeromonas veronii (MCBP), which has affinity for mucosal constituents such as mucin, lactoferrin, immunoglobulins and collagen, spotted sand bass (Paralabrax maculatofasciatus) were orally immunised either with soluble MCBP, adjuvant-conjugated MCBP or immobilised MCBP on latex microspheres. The results suggest that the MCBP is capable of eliciting protective immunity againstA. veronii infections when administered orally. The highest mucosal immune response was elicited in fish immunised with MCBP covalently linked to cholera toxin B subunit (CTB) or to Escherichia coli heat-labile toxin (hLT). MCBP-CTB was found to elicit immunoprotection against a challenge with live Aeromonas cells with a relative percent survival of almost 70% and without the expression of the severe histopathological alterations induced by A. veronii.

Original languageEnglish
Pages (from-to)115-126
Number of pages12
JournalFish and Shellfish Immunology
Volume11
Issue number2
DOIs
StatePublished - Feb 2001
Externally publishedYes

Keywords

  • Aeromonas veronii
  • Fish immunology
  • Lectins
  • Mucosal immunity
  • Paralabrax maculatofasciatus
  • Spotted sand bass

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