Abstract
Objective: To develop N-(levodopa) chitosan derivatives through click chemistry to study their effect in brain cells. Significance: This study presents a proof-of-concept that macromolecules such as N-(Levodopa) chitosan derivatives traverse brain cell membranes and induce biomedical functionalities. Methods: Through click chemistry, we developed N-(levodopa) chitosan derivatives. They were physically and chemically characterized by FT-IR, 1H-NMR, TGA and Dynamic Light Scattering analyses. Solution and nanoparticles of N-(levodopa) chitosan derivatives were tested in primary cell cultures from the postnatal rat olfactory bulb, substantia nigra and corpus callosum. Ca2+ imaging and UPLC experiments were used to investigate if the biomaterial modulated the brain cell physiology. Results: N-(levodopa) chitosan derivatives induced intracellular Ca2+ responses in primary cell cultures of the rat brain. UPLC experiments indicated that levodopa attached to chitosan was converted into dopamine by brain cells. Conclusion: The present study shows that N-(levodopa) chitosan may be useful to develop new treatment strategies, which could serve as molecular reservoirs of biomedical drugs to treat degenerative disorders of the nervous system.
Original language | English |
---|---|
Pages (from-to) | 439-447 |
Number of pages | 9 |
Journal | Drug Development and Industrial Pharmacy |
Volume | 49 |
Issue number | 7 |
DOIs | |
State | Published - 2023 |
Bibliographical note
Funding Information:This work was supported by Universidad de Sonora and CIAD. The authors thank Karla Zavalza Ortega, Bertha Pacheco Moreno, and Monica Villegas Ochoa for their technical assistance.
Publisher Copyright:
© 2023 Informa UK Limited, trading as Taylor & Francis Group.
Keywords
- Biomaterials
- biogenic amines
- brain disorders
- calcium and cellular physiology
- click and green chemistry
- dementia