Nanoscale Changes on RBC Membrane Induced by Storage and Ionizing Radiation: A Mini-Review

Andrea M. López-Canizales, Aracely Angulo-Molina*, Adriana Garibay-Escobar, Erika Silva-Campa, Miguel A. Mendez-Rojas, Karla Santacruz-Gómez, Mónica Acosta-Elías, Beatriz Castañeda-Medina, Diego Soto-Puebla, Osiris Álvarez-Bajo, Alexel Burgara-Estrella, Martín Pedroza-Montero

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

3 Scopus citations

Abstract

The storage lesions and the irradiation of blood cellular components for medical procedures in blood banks are events that may induce nanochanges in the membrane of red blood cells (RBCs). Alterations, such as the formation of pores and vesicles, reduce flexibility and compromise the overall erythrocyte integrity. This review discusses the alterations on erythrocytic lipid membrane bilayer through their characterization by confocal scanning microscopy, Raman, scanning electron microscopy, and atomic force microscopy techniques. The interrelated experimental results may address and shed light on the correlation of biomechanical and biochemical transformations induced in the membrane and cytoskeleton of stored and gamma-irradiated RBC. To highlight the main advantages of combining these experimental techniques simultaneously or sequentially, we discuss how those outcomes observed at micro- and nanoscale cell levels are useful as biomarkers of cell aging and storage damage.

Original languageEnglish
Article number669455
JournalFrontiers in Physiology
Volume12
DOIs
StatePublished - 4 Jun 2021
Externally publishedYes

Bibliographical note

Funding Information:
This research has received funding from CONACYT (Mexico) under Contract PDCPN2014-01/248982 and fellowship AL-C.

Publisher Copyright:
© Copyright © 2021 López-Canizales, Angulo-Molina, Garibay-Escobar, Silva-Campa, Mendez-Rojas, Santacruz-Gómez, Acosta-Elías, Castañeda-Medina, Soto-Puebla, Álvarez-Bajo, Burgara-Estrella and Pedroza-Montero.

Keywords

  • atomic force microscopy
  • blood storage
  • confocal microscopy
  • ionizing radiation
  • nanoalterations
  • Raman
  • RBC membrane
  • scanning electron microscopy

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