TY - JOUR
T1 - Nanoscale Changes on RBC Membrane Induced by Storage and Ionizing Radiation
T2 - A Mini-Review
AU - López-Canizales, Andrea M.
AU - Angulo-Molina, Aracely
AU - Garibay-Escobar, Adriana
AU - Silva-Campa, Erika
AU - Mendez-Rojas, Miguel A.
AU - Santacruz-Gómez, Karla
AU - Acosta-Elías, Mónica
AU - Castañeda-Medina, Beatriz
AU - Soto-Puebla, Diego
AU - Álvarez-Bajo, Osiris
AU - Burgara-Estrella, Alexel
AU - Pedroza-Montero, Martín
N1 - Publisher Copyright:
© Copyright © 2021 López-Canizales, Angulo-Molina, Garibay-Escobar, Silva-Campa, Mendez-Rojas, Santacruz-Gómez, Acosta-Elías, Castañeda-Medina, Soto-Puebla, Álvarez-Bajo, Burgara-Estrella and Pedroza-Montero.
PY - 2021/6/4
Y1 - 2021/6/4
N2 - The storage lesions and the irradiation of blood cellular components for medical procedures in blood banks are events that may induce nanochanges in the membrane of red blood cells (RBCs). Alterations, such as the formation of pores and vesicles, reduce flexibility and compromise the overall erythrocyte integrity. This review discusses the alterations on erythrocytic lipid membrane bilayer through their characterization by confocal scanning microscopy, Raman, scanning electron microscopy, and atomic force microscopy techniques. The interrelated experimental results may address and shed light on the correlation of biomechanical and biochemical transformations induced in the membrane and cytoskeleton of stored and gamma-irradiated RBC. To highlight the main advantages of combining these experimental techniques simultaneously or sequentially, we discuss how those outcomes observed at micro- and nanoscale cell levels are useful as biomarkers of cell aging and storage damage.
AB - The storage lesions and the irradiation of blood cellular components for medical procedures in blood banks are events that may induce nanochanges in the membrane of red blood cells (RBCs). Alterations, such as the formation of pores and vesicles, reduce flexibility and compromise the overall erythrocyte integrity. This review discusses the alterations on erythrocytic lipid membrane bilayer through their characterization by confocal scanning microscopy, Raman, scanning electron microscopy, and atomic force microscopy techniques. The interrelated experimental results may address and shed light on the correlation of biomechanical and biochemical transformations induced in the membrane and cytoskeleton of stored and gamma-irradiated RBC. To highlight the main advantages of combining these experimental techniques simultaneously or sequentially, we discuss how those outcomes observed at micro- and nanoscale cell levels are useful as biomarkers of cell aging and storage damage.
KW - RBC membrane
KW - Raman
KW - atomic force microscopy
KW - blood storage
KW - confocal microscopy
KW - ionizing radiation
KW - nanoalterations
KW - scanning electron microscopy
UR - http://www.scopus.com/inward/record.url?scp=85108235892&partnerID=8YFLogxK
U2 - 10.3389/fphys.2021.669455
DO - 10.3389/fphys.2021.669455
M3 - Artículo de revisión
AN - SCOPUS:85108235892
SN - 1664-042X
VL - 12
JO - Frontiers in Physiology
JF - Frontiers in Physiology
M1 - 669455
ER -