Native type IV collagen induces cell migration through a CD9 and DDR1-dependent pathway in MDA-MB-231 breast cancer cells

Luis Castro-Sanchez, Adriana Soto-Guzman, Napoleon Navarro-Tito, Raul Martinez-Orozco, Eduardo Perez Salazar

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

30 Citations (Scopus)

Abstract

CD9 is a member of the tetraspanin family and is widely expressed in the plasma membrane of several cell types as well as malignant cells. CD9 associates with a number of transmembrane proteins, which facilitates biological processes, including cell signaling, adhesion, migration and proliferation. DDR1 is activated by native type IV collagen and overexpressed in human breast cancer. Type IV collagen is the main component of basement membranes, and may interact with cell surface biomolecules, promoting adhesion and motility. However, the role of DDR1 and type IV collagen in the regulation of CD9-cell surface levels and migration in breast cancer cells has not been studied in detail. We demonstrate here that native type IV collagen induces a transient increase of CD9-cell surface levels through a DDR1-dependent pathway in MDA-MB-231 breast cancer cells, as revealed by flow cytometry and Western blotting using specific antibodies that recognize CD9. In contrast, type IV collagen does not induce any increase of CD9-cell surface levels in the mammary non-tumorigenic epithelial cells MCF10A and MCF12A. Transient increase of CD9-cell surface levels is coupled with clathrin-mediated endocytosis and it is dependent of DDR1 expression. In addition, type IV collagen induces cell migration through a DDR1 and CD9-dependent pathway. In summary, our data demonstrate, for the first time, that native type IV collagen induces a transient increase of CD9-cell surface levels and cell migration through a DDR1 and CD9-dependent pathway in MDA-MB-231 breast cancer cells. © 2010 Elsevier GmbH.
Original languageSpanish (Mexico)
Title of host publicationEuropean Journal of Cell Biology
Pages843-852
Number of pages10
DOIs
StatePublished - Nov 2010

Publication series

NameEuropean Journal of Cell Biology
Volume89

Cite this

Castro-Sanchez, L., Soto-Guzman, A., Navarro-Tito, N., Martinez-Orozco, R., & Salazar, E. P. (2010). Native type IV collagen induces cell migration through a CD9 and DDR1-dependent pathway in MDA-MB-231 breast cancer cells. In European Journal of Cell Biology (pp. 843-852). (European Journal of Cell Biology; Vol. 89). https://doi.org/10.1016/j.ejcb.2010.07.004
Castro-Sanchez, Luis ; Soto-Guzman, Adriana ; Navarro-Tito, Napoleon ; Martinez-Orozco, Raul ; Salazar, Eduardo Perez. / Native type IV collagen induces cell migration through a CD9 and DDR1-dependent pathway in MDA-MB-231 breast cancer cells. European Journal of Cell Biology. 2010. pp. 843-852 (European Journal of Cell Biology).
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abstract = "CD9 is a member of the tetraspanin family and is widely expressed in the plasma membrane of several cell types as well as malignant cells. CD9 associates with a number of transmembrane proteins, which facilitates biological processes, including cell signaling, adhesion, migration and proliferation. DDR1 is activated by native type IV collagen and overexpressed in human breast cancer. Type IV collagen is the main component of basement membranes, and may interact with cell surface biomolecules, promoting adhesion and motility. However, the role of DDR1 and type IV collagen in the regulation of CD9-cell surface levels and migration in breast cancer cells has not been studied in detail. We demonstrate here that native type IV collagen induces a transient increase of CD9-cell surface levels through a DDR1-dependent pathway in MDA-MB-231 breast cancer cells, as revealed by flow cytometry and Western blotting using specific antibodies that recognize CD9. In contrast, type IV collagen does not induce any increase of CD9-cell surface levels in the mammary non-tumorigenic epithelial cells MCF10A and MCF12A. Transient increase of CD9-cell surface levels is coupled with clathrin-mediated endocytosis and it is dependent of DDR1 expression. In addition, type IV collagen induces cell migration through a DDR1 and CD9-dependent pathway. In summary, our data demonstrate, for the first time, that native type IV collagen induces a transient increase of CD9-cell surface levels and cell migration through a DDR1 and CD9-dependent pathway in MDA-MB-231 breast cancer cells. {\circledC} 2010 Elsevier GmbH.",
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Castro-Sanchez, L, Soto-Guzman, A, Navarro-Tito, N, Martinez-Orozco, R & Salazar, EP 2010, Native type IV collagen induces cell migration through a CD9 and DDR1-dependent pathway in MDA-MB-231 breast cancer cells. in European Journal of Cell Biology. European Journal of Cell Biology, vol. 89, pp. 843-852. https://doi.org/10.1016/j.ejcb.2010.07.004

Native type IV collagen induces cell migration through a CD9 and DDR1-dependent pathway in MDA-MB-231 breast cancer cells. / Castro-Sanchez, Luis; Soto-Guzman, Adriana; Navarro-Tito, Napoleon; Martinez-Orozco, Raul; Salazar, Eduardo Perez.

European Journal of Cell Biology. 2010. p. 843-852 (European Journal of Cell Biology; Vol. 89).

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

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AU - Castro-Sanchez, Luis

AU - Soto-Guzman, Adriana

AU - Navarro-Tito, Napoleon

AU - Martinez-Orozco, Raul

AU - Salazar, Eduardo Perez

PY - 2010/11

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N2 - CD9 is a member of the tetraspanin family and is widely expressed in the plasma membrane of several cell types as well as malignant cells. CD9 associates with a number of transmembrane proteins, which facilitates biological processes, including cell signaling, adhesion, migration and proliferation. DDR1 is activated by native type IV collagen and overexpressed in human breast cancer. Type IV collagen is the main component of basement membranes, and may interact with cell surface biomolecules, promoting adhesion and motility. However, the role of DDR1 and type IV collagen in the regulation of CD9-cell surface levels and migration in breast cancer cells has not been studied in detail. We demonstrate here that native type IV collagen induces a transient increase of CD9-cell surface levels through a DDR1-dependent pathway in MDA-MB-231 breast cancer cells, as revealed by flow cytometry and Western blotting using specific antibodies that recognize CD9. In contrast, type IV collagen does not induce any increase of CD9-cell surface levels in the mammary non-tumorigenic epithelial cells MCF10A and MCF12A. Transient increase of CD9-cell surface levels is coupled with clathrin-mediated endocytosis and it is dependent of DDR1 expression. In addition, type IV collagen induces cell migration through a DDR1 and CD9-dependent pathway. In summary, our data demonstrate, for the first time, that native type IV collagen induces a transient increase of CD9-cell surface levels and cell migration through a DDR1 and CD9-dependent pathway in MDA-MB-231 breast cancer cells. © 2010 Elsevier GmbH.

AB - CD9 is a member of the tetraspanin family and is widely expressed in the plasma membrane of several cell types as well as malignant cells. CD9 associates with a number of transmembrane proteins, which facilitates biological processes, including cell signaling, adhesion, migration and proliferation. DDR1 is activated by native type IV collagen and overexpressed in human breast cancer. Type IV collagen is the main component of basement membranes, and may interact with cell surface biomolecules, promoting adhesion and motility. However, the role of DDR1 and type IV collagen in the regulation of CD9-cell surface levels and migration in breast cancer cells has not been studied in detail. We demonstrate here that native type IV collagen induces a transient increase of CD9-cell surface levels through a DDR1-dependent pathway in MDA-MB-231 breast cancer cells, as revealed by flow cytometry and Western blotting using specific antibodies that recognize CD9. In contrast, type IV collagen does not induce any increase of CD9-cell surface levels in the mammary non-tumorigenic epithelial cells MCF10A and MCF12A. Transient increase of CD9-cell surface levels is coupled with clathrin-mediated endocytosis and it is dependent of DDR1 expression. In addition, type IV collagen induces cell migration through a DDR1 and CD9-dependent pathway. In summary, our data demonstrate, for the first time, that native type IV collagen induces a transient increase of CD9-cell surface levels and cell migration through a DDR1 and CD9-dependent pathway in MDA-MB-231 breast cancer cells. © 2010 Elsevier GmbH.

KW - Breast cancer

KW - CD9

KW - DDR1

KW - Type IV Collagen

UR - http://www.mendeley.com/research/native-type-iv-collagen-induces-cell-migration-through-cd9-ddr1dependent-pathway-mdamb231-breast-can-1

U2 - 10.1016/j.ejcb.2010.07.004

DO - 10.1016/j.ejcb.2010.07.004

M3 - Capítulo

SN - 1618-1298 (Electronic)\r0171-9335 (Linking)

T3 - European Journal of Cell Biology

SP - 843

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BT - European Journal of Cell Biology

ER -

Castro-Sanchez L, Soto-Guzman A, Navarro-Tito N, Martinez-Orozco R, Salazar EP. Native type IV collagen induces cell migration through a CD9 and DDR1-dependent pathway in MDA-MB-231 breast cancer cells. In European Journal of Cell Biology. 2010. p. 843-852. (European Journal of Cell Biology). https://doi.org/10.1016/j.ejcb.2010.07.004