Novel angiotensin I-converting enzyme inhibitory peptides produced in fermented milk by specific wild Lactococcus lactis strains

J. C. Rodríguez-Figueroa, A. F. González-Córdova, M. J. Torres-Llanez, H. S. Garcia, B. Vallejo-Cordoba*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

The ability of specific wild Lactococcus lactis strains to hydrolyze milk proteins to release angiotensin I-converting enzyme (ACE) inhibitory peptides was evaluated. The peptide profiles were obtained from the <3. kDa water-soluble extract and subsequently fractionated by reversed-phase HPLC. The fractions with the lowest half-maximal inhibitory concentration estimated values (peptide concentration necessary to inhibit ACE activity by 50%) were Lc. lactis NRRL B-50571 fraction (F)1 (0.034 ± 0.002 μg/mL; mean ± SD) and Lc. lactis NRRL B-50572B F 0005 (0.041 ± 0.003 μg/mL; mean ± SD). All peptide fractions were analyzed by reversed-phase HPLC tandem mass spectrometry. Twenty-one novel peptide sequences associated with ACE inhibitory (ACEI) activity were identified. Several novel ACEI peptides presented peptides encrypted with proven hypotensive activity. In conclusion, specific wild Lc. lactis strains were able to hydrolyze milk proteins to generate potent ACEI peptides. However, further studies are necessary to find out the relationship between Lc. lactis strain proteolytic systems and their ability to biogenerate hypotensive peptides.

Original languageEnglish
Pages (from-to)5536-5543
Number of pages8
JournalJournal of Dairy Science
Volume95
Issue number10
DOIs
StatePublished - Oct 2012
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by the Mexican National Council of Science and Technology (CONACyT) research grant CB-2009-1 134295 . We thank María del Carmen Estrada-Montoya (CIAD, Sonora, Mexico) for her technical support. Authors J. C. Rodríguez-Figueroa, A. F. González-Córdova, M. J. Torres-Llanez, and B. Vallejo-Cordoba express their gratitude to the Mexican Coca-Cola Co. (Asociación de Embotelladoras Mexicanas de Coca-Cola, A.C., Col. Polanco, México) and CONACyT for granting the 2011 National Prize on Food Science and Technology to part of this study.

Keywords

  • Angiotensin-converting enzyme inhibitory peptide
  • Fermented milk
  • Lactococcus lactis

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