Novel synthesis of core-shell silica nanoparticles for the capture of low molecular weight proteins and peptides

Sergio G. Hernandez-Leon, Jose Andre I. Sarabia-Sainz, Gabriela Ramos Clamont Montfort, Ana M. Guzman-Partida, Maria Del Refugio Robles-Burgueño, Luz Vazquez-Moreno*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Silica nanoparticles were functionalized with immobilized molecular bait, Cibacron Blue, and a porous polymeric bis-acrylamide shell. These nanoparticles represent a new alternative to capture low molecular weight (LMW) proteins/peptides, that might be potential biomarkers. Functionalized core-shell silica nanoparticles (FCSNP) presented a size distribution of 243.9 - 11.6 nmand an estimated surface charge of 38.1 - 0.9 mV. The successful attachment of compounds at every stage of synthesis was evidenced by ATR-FTIR. The capture of model peptides was determined by mass spectrometry, indicating that only the peptide with a long sequence of hydrophobic amino acids (alpha zein 34-mer) interacted with the molecular bait. FCSNP excluded the high molecular weight protein (HMW), BSA, and captured LMWproteins (myoglobin and aprotinin), as evidenced by SDS-PAGE. Functionalization of nanoparticles with Cibacron Blue was crucial to capture these molecules. FCSNP were stable after twelve months of storage and maintained a capacity of 3.1-3.4 g/mg.

Original languageEnglish
Article number1712
JournalMolecules
Volume22
Issue number10
DOIs
StatePublished - Oct 2017

Bibliographical note

Publisher Copyright:
© 2017 by the authors.

Keywords

  • Cibacron blue
  • Functionalized core-shell silica nanoparticles
  • High molecular weight proteins
  • Hydrophobic
  • Low molecular weight proteins/peptides

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