Oleic acid promotes migration on MDA-MB-231 breast cancer cells through an arachidonic acid-dependent pathway

Napoleon Navarro-Tito, Adriana Soto-Guzman, Luis Castro-Sanchez, Raul Martinez-Orozco, Eduardo Perez Salazar

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

52 Scopus citations

Abstract

An association between dietary fatty, obesity and an increased risk of developing breast cancer has been suggested. In breast cancer cells, free fatty acids (FFAs) mediate biological effects including cell proliferation and ERK1/2 activation. However, the contribution of FFAs to tumor progression and metastasis through the regulation of cell migration has not been studied. We demonstrated here that stimulation on MDA-MB-231 breast cancer cells with oleic acid (OA) promotes an increase in focal adhesion kinase (FAK) phosphorylation, as revealed by site-specific antibodies that recognize the phosphorylation state of FAK at tyrosine-397 (Tyr-397), Tyr-577 and in vitro kinase assays. OA also promotes the migration of MDA-MB-231 cells. Treatment with Gi/Go proteins, phospholipase C (PLC), lipoxygenases (LOXs) and Src inhibitor prevents FAK phosphorylation and cell migration. In summary, our findings delineate a new signal transduction pathway, where OA mediates the production of arachidonic acid (AA), and then AA metabolites mediate FAK phosphorylation and cell migration in MDA-MB-231 breast cancer cells. © 2009 Elsevier Ltd. All rights reserved.
Original languageEnglish
Title of host publicationInternational Journal of Biochemistry and Cell Biology
Pages306-317
Number of pages12
DOIs
StatePublished - Feb 2010

Publication series

NameInternational Journal of Biochemistry and Cell Biology
Volume42

Keywords

  • Arachidonic acid
  • Breast cancer
  • FAK
  • LOX
  • Oleic acid

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