TY - JOUR
T1 - Optimal Immobilization of Acidic Proteases from Monterey Sardine (Sardinops sagax caeurelea) on Partially Deacetylated Chitin from Shrimp Head Waste
AU - Salazar-Leyva, Jesus Aaron
AU - Lizardi-Mendoza, Jaime
AU - Ramirez-Suarez, Juan Carlos
AU - Valenzuela-Soto, Elisa Miriam
AU - Ezquerra-Brauer, Josafat Marina
AU - Castillo-Yañez, Francisco Javier
AU - Lugo-Sanchez, Maria Elena
AU - Garcia-Sanchez, Guillermina
AU - Carvallo-Ruiz, Maria Gisela
AU - Pacheco-Aguilar, Ramon
N1 - Publisher Copyright:
© 2016 Taylor & Francis.
PY - 2016/10/2
Y1 - 2016/10/2
N2 - Response surface methodology was employed to optimize the immobilization yield of acidic proteases from Monterey sardine (Sardinops sagax caeurelea) using partially deacetylated chitin as immobilization support. A rotatable central composite design was applied to evaluate the effects of immobilization conditions such as enzyme loading (X1), immobilization pH (X2), and tripolyphosphate concentration (X3) on the immobilization yield. The analysis of variance revealed that the established model was significant (p < 0.05), and the adjustment of the quadratic model with the experimental data was satisfactory. Under optimal conditions (X1 = 0.05 mg/mL, X2 = 3.16, and X3 = 0.75%), an immobilization yield of 79.1% was achieved; a value that was in accordance with the predicted one.
AB - Response surface methodology was employed to optimize the immobilization yield of acidic proteases from Monterey sardine (Sardinops sagax caeurelea) using partially deacetylated chitin as immobilization support. A rotatable central composite design was applied to evaluate the effects of immobilization conditions such as enzyme loading (X1), immobilization pH (X2), and tripolyphosphate concentration (X3) on the immobilization yield. The analysis of variance revealed that the established model was significant (p < 0.05), and the adjustment of the quadratic model with the experimental data was satisfactory. Under optimal conditions (X1 = 0.05 mg/mL, X2 = 3.16, and X3 = 0.75%), an immobilization yield of 79.1% was achieved; a value that was in accordance with the predicted one.
KW - Response surface methodology
KW - acidic proteases
KW - chitin
KW - immobilization
UR - http://www.scopus.com/inward/record.url?scp=84990244102&partnerID=8YFLogxK
U2 - 10.1080/10498850.2015.1033583
DO - 10.1080/10498850.2015.1033583
M3 - Artículo
SN - 1049-8850
VL - 25
SP - 1144
EP - 1154
JO - Journal of Aquatic Food Product Technology
JF - Journal of Aquatic Food Product Technology
IS - 7
ER -