TY - JOUR
T1 - Optimization of Carvacrol Nanoemulsion for the Incorporation in Pectin Membranes
T2 - Influence on Their Load Capacity, Microstructure and Antibacterial Properties
AU - Beltrán, O.
AU - Luna, M.
AU - Valbuena-Gregorio, E.
AU - Valdez-Melchor, R. G.
AU - Burruel-Ibarra, S. E.
AU - Quihui-Cota, L.
AU - Ruiz-Cruz, S.
AU - Juárez, J.
AU - López-Mata, M. A.
N1 - Publisher Copyright:
© 2022 Universidade Federal de Sao Carlos. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Interest in developing novel wound dressings with antibacterial properties elaborated from natural sources continues to grow. In this study, a Tween-80 (T80)-stabilized carvacrol (CAR) emulsion was incorporated into pectin (PEC) membranes at 0 (control), 0.25, 0.50, and 1.00% (v/v). Membranes were obtained by the dry-casting method, characterized by scanning electron microscopy, infrared spectroscopy, and CAR retention (HPLC), and tested for antibacterial activity. The retention percentage of CAR in the membranes ranged from 9.1-13.9%. Infrared spectra analysis indicated changes in the hydrogen bonds of the membranes that suggest an interaction between the polymer matrix and the CAR:T80 emulsion. Microstructural analysis of the membranes showed the presence of hole-like features on the surface (≈ 4-6 μm diameter) that indicate entrapment of the micelles in the matrix (microcapsules). The PEC-CAR membranes exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus, two pathogens commonly associated with wounds and intra-hospital infections.
AB - Interest in developing novel wound dressings with antibacterial properties elaborated from natural sources continues to grow. In this study, a Tween-80 (T80)-stabilized carvacrol (CAR) emulsion was incorporated into pectin (PEC) membranes at 0 (control), 0.25, 0.50, and 1.00% (v/v). Membranes were obtained by the dry-casting method, characterized by scanning electron microscopy, infrared spectroscopy, and CAR retention (HPLC), and tested for antibacterial activity. The retention percentage of CAR in the membranes ranged from 9.1-13.9%. Infrared spectra analysis indicated changes in the hydrogen bonds of the membranes that suggest an interaction between the polymer matrix and the CAR:T80 emulsion. Microstructural analysis of the membranes showed the presence of hole-like features on the surface (≈ 4-6 μm diameter) that indicate entrapment of the micelles in the matrix (microcapsules). The PEC-CAR membranes exhibited antibacterial activity against Escherichia coli and Staphylococcus aureus, two pathogens commonly associated with wounds and intra-hospital infections.
KW - antibacterial
KW - carvacrol
KW - emulsion
KW - membrane
KW - pectin
UR - http://www.scopus.com/inward/record.url?scp=85139077464&partnerID=8YFLogxK
U2 - 10.1590/1980-5373-MR-2021-0534
DO - 10.1590/1980-5373-MR-2021-0534
M3 - Artículo
AN - SCOPUS:85139077464
SN - 1516-1439
VL - 25
JO - Materials Research
JF - Materials Research
M1 - e20210534
ER -