Optimization of structured diacylglycerols production containing ω-3 fatty acids via enzyme-catalysed glycerolysis of fish oil

Kelhy Miranda, Ramiro Baeza-Jiménez, Juan A. Noriega-Rodríguez, Hugo S. García, Cristina Otero

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The main objective of the present work was the production of structured diacylglycerols (DAG) by Novozym 435-catalysed glycerolysis of n-3 PUFA rich fish oil. Different enzyme loads (5, 7, 10, 13 and 15 %, with respect to the total weight of substrates), temperatures (40, 44, 50, 56 and 60 °C) and molar ratios of substrates (1, 1. 4, 2, 2. 6 and 3, oil/glycerol) were explored by the response surface methodology in order to optimize the reaction system. Temperature showed the highest significant effect (P < 0. 05) on the glycerolysis reaction. Good agreement on the experimental data and the second-order polynomial regression was obtained (R2 = 0. 85). Optimal conditions for the production of DAG were established by the model using 15 % enzyme load at 60 °C for a 2:1 (oil/glycerol) substrates molar ratio, reaching a 50 % yield, with 89. 37 % n-3 PUFA content, namely, EPA (11. 32 %), DPA (8. 34 %) and DHA (69. 71 %). © 2013 Springer-Verlag Berlin Heidelberg.
Original languageAmerican English
Pages (from-to)435-440
Number of pages6
JournalEuropean Food Research and Technology
DOIs
StatePublished - 9 Jan 2013

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