Optimization of the Lipase Catalyzed Production of Structured Acylglycerols With Polyunsaturated Fatty Acids Isolated From Sardine Oil

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Abstract

<p>In the present work, direct enzyme-catalyzed esterification of n-3 polyunsaturated fatty acids (n-3 PUFA) isolated from sardine oil was optimized to obtain structured acyglycerols. A n-3 PUFA concentrate was prepared by urea crystallization of refined sardine oil and esterification was carried out mixing free fatty acids and glycerol at different molar ratios (<em>M</em> = 0.48, 1.5, 3.0, 4.5 and 5.52 mol/mol), using an immobilized lipase preparation from <em>Candida antarctica</em> (NV-435) at different temperatures (<em>T</em> = 38, 45, 55, 65 and 72 °C) and reaction times (<em>t</em> = 0.7, 2.75, 5.75, 8.75 and 10.8 h) in a rotatable central composition design. The degree of esterification was determined by analysis of the acylglycerides produced, using liquid chromatography (HPLC-ELSD). Optimization by response surface methodology (RSM) showed that in order to obtain higher esterification levels of n-3 PUFA to glycerol (99.5%), a molar ratio of 1.3 mol n-3 PUFA/mol glycerol, time 8.3 h and temperature 38 °C, are required. However, results of this work show that it is possible to drive the reaction to any determined product (MAG, DAG or TAG) by modifying the reaction conditions.</p>

Original languageAmerican English
Pages (from-to)97-105
Number of pages9
JournalJournal of Food Research
Volume2
Issue number6
DOIs
StatePublished - 19 Nov 2013

Cite this

@article{4e4ed7c4bda44395b66da84c1e926642,
title = "Optimization of the Lipase Catalyzed Production of Structured Acylglycerols With Polyunsaturated Fatty Acids Isolated From Sardine Oil",
abstract = "In the present work, direct enzyme-catalyzed esterification of n-3 polyunsaturated fatty acids (n-3 PUFA) isolated from sardine oil was optimized to obtain structured acyglycerols. A n-3 PUFA concentrate was prepared by urea crystallization of refined sardine oil and esterification was carried out mixing free fatty acids and glycerol at different molar ratios (M = 0.48, 1.5, 3.0, 4.5 and 5.52 mol/mol), using an immobilized lipase preparation from Candida antarctica (NV-435) at different temperatures (T = 38, 45, 55, 65 and 72 °C) and reaction times (t = 0.7, 2.75, 5.75, 8.75 and 10.8 h) in a rotatable central composition design. The degree of esterification was determined by analysis of the acylglycerides produced, using liquid chromatography (HPLC-ELSD). Optimization by response surface methodology (RSM) showed that in order to obtain higher esterification levels of n-3 PUFA to glycerol (99.5{\%}), a molar ratio of 1.3 mol n-3 PUFA/mol glycerol, time 8.3 h and temperature 38 °C, are required. However, results of this work show that it is possible to drive the reaction to any determined product (MAG, DAG or TAG) by modifying the reaction conditions.",
author = "Noriega-Rodriguez, {Juan Antonio} and Esther Carrillo-Perez and Nohemi Gamez-Meza and Medina-Juarez, {Luis A.} and Ramiro Baeza-Jimenez and Garcia, {Hugo S.}",
year = "2013",
month = "11",
day = "19",
doi = "10.5539/jfr.v2n6p97",
language = "American English",
volume = "2",
pages = "97--105",
journal = "Journal of Food Research",
issn = "1927-0895",
number = "6",

}

TY - JOUR

T1 - Optimization of the Lipase Catalyzed Production of Structured Acylglycerols With Polyunsaturated Fatty Acids Isolated From Sardine Oil

AU - Noriega-Rodriguez, Juan Antonio

AU - Carrillo-Perez, Esther

AU - Gamez-Meza, Nohemi

AU - Medina-Juarez, Luis A.

AU - Baeza-Jimenez, Ramiro

AU - Garcia, Hugo S.

PY - 2013/11/19

Y1 - 2013/11/19

N2 - In the present work, direct enzyme-catalyzed esterification of n-3 polyunsaturated fatty acids (n-3 PUFA) isolated from sardine oil was optimized to obtain structured acyglycerols. A n-3 PUFA concentrate was prepared by urea crystallization of refined sardine oil and esterification was carried out mixing free fatty acids and glycerol at different molar ratios (M = 0.48, 1.5, 3.0, 4.5 and 5.52 mol/mol), using an immobilized lipase preparation from Candida antarctica (NV-435) at different temperatures (T = 38, 45, 55, 65 and 72 °C) and reaction times (t = 0.7, 2.75, 5.75, 8.75 and 10.8 h) in a rotatable central composition design. The degree of esterification was determined by analysis of the acylglycerides produced, using liquid chromatography (HPLC-ELSD). Optimization by response surface methodology (RSM) showed that in order to obtain higher esterification levels of n-3 PUFA to glycerol (99.5%), a molar ratio of 1.3 mol n-3 PUFA/mol glycerol, time 8.3 h and temperature 38 °C, are required. However, results of this work show that it is possible to drive the reaction to any determined product (MAG, DAG or TAG) by modifying the reaction conditions.

AB - In the present work, direct enzyme-catalyzed esterification of n-3 polyunsaturated fatty acids (n-3 PUFA) isolated from sardine oil was optimized to obtain structured acyglycerols. A n-3 PUFA concentrate was prepared by urea crystallization of refined sardine oil and esterification was carried out mixing free fatty acids and glycerol at different molar ratios (M = 0.48, 1.5, 3.0, 4.5 and 5.52 mol/mol), using an immobilized lipase preparation from Candida antarctica (NV-435) at different temperatures (T = 38, 45, 55, 65 and 72 °C) and reaction times (t = 0.7, 2.75, 5.75, 8.75 and 10.8 h) in a rotatable central composition design. The degree of esterification was determined by analysis of the acylglycerides produced, using liquid chromatography (HPLC-ELSD). Optimization by response surface methodology (RSM) showed that in order to obtain higher esterification levels of n-3 PUFA to glycerol (99.5%), a molar ratio of 1.3 mol n-3 PUFA/mol glycerol, time 8.3 h and temperature 38 °C, are required. However, results of this work show that it is possible to drive the reaction to any determined product (MAG, DAG or TAG) by modifying the reaction conditions.

UR - http://www.ccsenet.org/journal/index.php/jfr/article/view/32145

UR - http://www.mendeley.com/research/optimization-lipase-catalyzed-production-structured-acylglycerols-polyunsaturated-fatty-acids-isolat

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