Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface hydrophobicity of cell-associated proteins extracted with 3 mol/L urea, revealing proteins with a significant hydrophobic profile.
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Fig. 2 Hypothetical model of the participation of charge interactions for iron-uptake and adhesion-colonization in H.p. infections, where hydrophobins may be involved in the stabilization of the iron-uptake mechanism, and lectin-like interactions may have a similar function in the host adhesion and colonization process This work was supported by the Center for Biological Research, CIBNOR (ABM-11). The first author was recipient of a scholarship (59291) from the Mexican Council of Science and Technology (CONACyT). We thank Tec. A.A. Cruz for assistance on chromatographic procedures.