Participation of cell-surface hydrophobins for hemin binding in Helicobacter pylori

E. Ruiz-Bustos; T. Wadström; F. Ascencio

doi:10.1007/s12223-011-0043-z

Participation of cell-surface hydrophobins for hemin binding in Helicobacter pylori

E. Ruiz-Bustos^*, T. Wadström, F. Ascencio

^*Corresponding author for this work

Departamento de Ciencias Químico-Biológicas

Research output: Contribution to journal › Article › peer-review

Abstract

Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface hydrophobicity of cell-associated proteins extracted with 3 mol/L urea, revealing proteins with a significant hydrophobic profile.

Original language	English
Pages (from-to)	241-245
Number of pages	5
Journal	Folia Microbiologica
Volume	56
Issue number	3
DOIs	https://doi.org/10.1007/s12223-011-0043-z
State	Published - May 2011

Bibliographical note

Funding Information:
Fig. 2 Hypothetical model of the participation of charge interactions for iron-uptake and adhesion-colonization in H.p. infections, where hydrophobins may be involved in the stabilization of the iron-uptake mechanism, and lectin-like interactions may have a similar function in the host adhesion and colonization process This work was supported by the Center for Biological Research, CIBNOR (ABM-11). The first author was recipient of a scholarship (59291) from the Mexican Council of Science and Technology (CONACyT). We thank Tec. A.A. Cruz for assistance on chromatographic procedures.

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title = "Participation of cell-surface hydrophobins for hemin binding in Helicobacter pylori",

abstract = "Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface hydrophobicity of cell-associated proteins extracted with 3 mol/L urea, revealing proteins with a significant hydrophobic profile.",

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note = "Funding Information: Fig. 2 Hypothetical model of the participation of charge interactions for iron-uptake and adhesion-colonization in H.p. infections, where hydrophobins may be involved in the stabilization of the iron-uptake mechanism, and lectin-like interactions may have a similar function in the host adhesion and colonization process This work was supported by the Center for Biological Research, CIBNOR (ABM-11). The first author was recipient of a scholarship (59291) from the Mexican Council of Science and Technology (CONACyT). We thank Tec. A.A. Cruz for assistance on chromatographic procedures.",

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doi = "10.1007/s12223-011-0043-z",

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AU - Ruiz-Bustos, E.

AU - Wadström, T.

AU - Ascencio, F.

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AB - Treatment of Helicobacter pylori cells with several chaotropic agents resulted in different degrees of inhibition in the binding of the bacteria to hemin and Congo-red dye. Polyanions also yielded a >50% inhibitory effect. Furthermore, hydrophobic interaction chromatography was used to determine the relative surface hydrophobicity of cell-associated proteins extracted with 3 mol/L urea, revealing proteins with a significant hydrophobic profile.

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JO - Folia Microbiologica

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ER -

Participation of cell-surface hydrophobins for hemin binding in Helicobacter pylori

Abstract

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