Perilipin Isoforms and PGC-1α Are Regulated Differentially in Rat Heart during Pregnancy-Induced Physiological Cardiac Hypertrophy

Jesús A. Rosas-Rodríguez, Adolfo Virgen-Ortíz, Enrico A. Ruiz, Rudy M. Ortiz, José G. Soñanez-Organis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background and Objectives: Perilipins 1–5 (PLIN) are lipid droplet-associated proteins that participate in regulating lipid storage and metabolism, and the PLIN5 isoform is known to form a nuclear complex with peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1α) to regulate lipid metabolism gene expression. However, the changes in PLIN isoforms’ expression in response to pregnancy-induced cardiac hypertrophy are not thoroughly studied. The aim of this study was to quantify the mRNA expression of PLIN isoforms and PGC-1α along with total triacylglycerol (TAG) and cholesterol levels during late pregnancy and the postpartum period in the rat left ventricle. Materials and Methods: Female Sprague-Dawley rats were divided into three groups: non-pregnant, late pregnancy, and postpartum. The mRNA and protein levels were evaluated using quantitative RT-PCR and Western blotting, respectively. TAG and total cholesterol content were evaluated using commercial colorimetric methods. Results: The expression of mRNAs for PLIN1, 2, and 5 increased during pregnancy and the postpartum period. PGC-1α mRNA and protein expression increased during pregnancy and the postpartum period. Moreover, TAG and total cholesterol increased during pregnancy and returned to basal levels after pregnancy. Conclusions: Our results demonstrate that pregnancy upregulates differentially the expression of PLIN isoforms along with PGC-1α, suggesting that together they might be involved in the regulation of the lipid metabolic shift induced by pregnancy.

Original languageEnglish
Article number1433
JournalMedicina (Lithuania)
Volume58
Issue number10
DOIs
StatePublished - Oct 2022

Bibliographical note

Funding Information:
This work was supported by the University of California Institute for Mexico and the United States, Mexico’s National Council for Science and Technology (UC MEXUS-CONACYT), [grant numbers CN-19-194], and the Universidad de Sonora [grant number USO513006176].

Publisher Copyright:
© 2022 by the authors.

Keywords

  • PGC-1α
  • gene expression
  • lipid metabolism
  • perilipin
  • pregnancy
  • protein abundance

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