Physicochemical and Microstructural Characteristics of Sulfated Polysaccharide from Marine Microalga

Diana Fimbres-Olivarria*, Jorge Marquez-Escalante*, Karla G. Martínez-Robinson, Valeria Miranda-Arizmendi, Yubia De Anda-Flores, Agustín Rascon-Chu, Francisco Brown-Bojorquez, Elizabeth Carvajal-Millan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Marine algae are a valuable source of polysaccharides. However, the information available on sulfated polysaccharides from microalgae is limited. Navicula sp. is a microalga present in the Sea of Cortez, of which little is known regarding their polysaccharides’ properties. This study investigated the physicochemical and microstructural characteristics of Navicula sp. sulfated polysaccharide (NSP). The Fourier transform infrared spectrum of NSP showed distinctive bands (1225 and 820 cm−1, assigned to S–O and C–O–S stretching, respectively), confirming the molecular identity. NSP registered molecular weight, intrinsic viscosity, a radius of gyration, and a hydrodynamic radius of 1650 kDa, 197 mL/g, 61 nm, and 36 nm, respectively. The zeta potential, electrophoretic mobility, conductivity, and diffusion coefficient of the molecule were −5.8 mV, −0.45 µm cm/s V, 0.70 mS/cm, and 2.9 × 10−9 cm2/s, respectively. The characteristic ratio and persistence length calculated for NSP were 4.2 and 1.3 nm, suggesting a nonstiff polysaccharide chain conformation. The Mark–Houwink–Sakurada α and K constants were 0.5 and 1.67 × 10−1, respectively, indicating a molecular random coil structure. NSP scanning electron microscopy revealed a rough and porous surface. Knowing these polysaccharides’ physicochemical and microstructural characteristics can be the starting point for elucidating their structure–function relationship as a valuable tool in advanced biomaterial design.

Original languageEnglish
Pages (from-to)527-537
Number of pages11
JournalAnalytica
Volume4
Issue number4
DOIs
StatePublished - Dec 2023

Bibliographical note

Publisher Copyright:
© 2023 by the authors.

Keywords

  • chain conformation
  • macromolecular characteristics
  • marine diatoms
  • multi-angle light scattering
  • scanning electron microscopy
  • structure–function relationship
  • sulfated polysaccharides

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