Physiological and immune responses of white shrimp (Litopenaeus vannamei) infected with necrotizing hepatopancreatitis bacterium

Luz Angélica Ávila-Villa, Diana Fimbres-Olivarria, Guillermina García-Sánchez, Teresa Gollas-Galván*, Jorge Hernández-López, Marcel Martínez-Porchas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

An eighteen day trial was performed to evaluate the effect of necrotizing hepatopancreatitis (NHP) disease on the physiological, nutritional and immunological status of adult white shrimp (Litopenaeus vannamei). Shrimp were divided into infected and non-infected groups; infected shrimp were fed with hepatopancreas homegenates positive to NHP bacterium, while non-infected shrimp were fed with healthy hepatopancreas. No significant differences were observed for protein, glucose, acylglicerides, cholesterol, ammonia and urea concentrations in haemolymph. However, lactate levels increased by 95% in infected shrimp compared to non-infected shrimp at 18th day post inoculation; similarly, nitrite levels in infected shrimp significantly raised by 141% and 66% at 15th and 18th days respectively. The total phenoloxidase and phenoloxidase activities of infected shrimp increased significantly at 7th day post inoculation, whereas no significant differences were detected for prophenoloxidase and total hemocyte count. The antibacterial activity decreased in infected shrimp at 15th day post inoculation. Total proteinase, trypsin and quimotrypsin activities increased after 15 days from inoculation in infected shrimp; however trypsin and quimotrypsin activities returned to their original levels after 18 days. Results suggest that shrimp maintained a homeostasis status in most of the monitored parameters, while others were modified and returned to their original levels, indicating that shrimp can withstand the disease during the first phase by performing different physiological and biochemical modifications in order to maintain homeostasis (general adaptation syndrome).

Original languageEnglish
Pages (from-to)14-19
Number of pages6
JournalAquaculture
Volume324-325
DOIs
StatePublished - 12 Jan 2012
Externally publishedYes

Keywords

  • Experimental infection
  • Immune status
  • Necrotizing hepatopancreatitis
  • Physiological status
  • Shrimp disease
  • Stress

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