Prevalence of Antibiotic-Resistant E. coli Strains in a Local Farm and Packing Facilities of Honeydew Melon in Hermosillo, Sonora, Mexico

Yessica Enciso-Martínez, Edwin Barrios-Villa, César O. Sepúlveda-Moreno, Manuel G. Ballesteros-Monrreal, Dora E. Valencia-Rivera, Gustavo A. González-Aguilar, Miguel A. Martínez-Téllez, Jesús Fernando Ayala-Zavala*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Pathogenic strains of Escherichia coli threaten public health due to their virulence factors and antibiotic resistance. Additionally, the virulence of this bacterium varies by region depending on environmental conditions, agricultural practices, and the use of antibiotics and disinfectants. However, there is limited research on the prevalence of antibiotic-resistant E. coli in agriculture. Therefore, this research aimed to determine the antibiotic resistance of E. coli isolated from the Honeydew melon production system in Hermosillo, Sonora, Mexico. Thirty-two E. coli strains were isolated from 445 samples obtained from irrigation water, harvested melons, the hands of packaging workers, boxes, and discarded melons. The resistance profile of the E. coli strains was carried out to 12 antibiotics used in antimicrobial therapeutics against this bacterium; a high level of resistance to ertapenem (100%) was detected, followed by meropenem (97%), and ampicillin (94%); 47% of the strains were classified as multidrug-resistant. It was possible to identify the prevalence of the extended-spectrum β-lactamase (ESBLs) gene blaTEM (15.6%), as well as the non-ESBL genes qepA (3.1%) and aac(6′)lb-cr (3.1%). The E. coli strains isolated from irrigation water were significantly associated with resistance to aztreonam, cefuroxime, amikacin, and sulfamethoxazole/trimethoprim. Irrigation water, packing workers’ hands, and discarded melons showed a higher prevalence of antibiotic-resistant, ESBL, and non-ESBL genes of E. coli strains in a farm and packing facility of Honeydew melon in Hermosillo, Sonora.

Original languageEnglish
Article number1789
JournalAntibiotics
Volume11
Issue number12
DOIs
StatePublished - Dec 2022

Bibliographical note

Funding Information:
The Consejo Nacional de Ciencia y Tecnología (CONACYT) is thanked for the scholarship awarded (932713) to Enciso-Martínez for doctoral studies. Consejo Nacional de Ciencia y Tecnología (CONACYT) granted funding for the project 319752 “Modelaje de la combinación de antibióticos convencionales y terpenos de aceites esenciales para inhibir la resistencia y factores de virulencia de bacterias patógenas alimentarias”.

Publisher Copyright:
© 2022 by the authors.

Keywords

  • antibiotics
  • food safety
  • multidrug-resistance bacteria
  • pathogenic strains

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