Producción de callogénesis en explantes de Vitex mollis Kunth recurso fitogenético de la selva baja caducifolia

Translated title of the contribution: Induction of callogenesis in explants of Vitex mollis Kunth plant genetic resource of the low deciduous forest

Alicia Álvarez-Martínez, Diana M. Mc Caughey-Espinoza*, Andrés Ochoa-Meza, Nydia E. Buitimea-Cantúa*, Cipriano Fuentes-Verduzco, Elisa Magaña-Barajas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Vitex mollis Kunth is a tree that belongs to the Lamiaceae family, and is found throughout Mexico. The objective of this work was to establish a methodology for obtaining callogenesis of Vitex mollis in vitro. The percentage of callogenesis in stem was 75%, in axillary bud and apical bud (85% and 80%, respectively) and leaf 60%. Callus growth with respect to height, evaluation time (4, 5 and 6 incubation periods) and concentrations of 1.0, 1.5 and 2.0 mgL–1 of IBA was better in axillary bud with 0.70, 1.79 and 2.60 mm and in apical bud 0.56, 1.22 and 2.33mm. The average callus height with 1.0, 1.5 and 2.0 mgL–1 of IBA in the axillary bud was 1.04 to 1.14 mm and in the apical bud 0.91 to 1.06 mm. The width of the callus in the axillary bud, apical bud and stem were the ones with the highest growth over the time evaluated. When evaluating the width of the callus with 0.5, 1.0, 1.5 and 2.0 mgL–1 of IBA, the average in the stem was 0.92, 1.04, 1.08 and 1.14 mm, axillary bud 1.14, 1.22, 1.40, and 1.50 mm, apical bud 0.97, 1.08, 1.13 and 1.16 mm, leaf in the concentrations of 1.0, 1.5 and 2.0 mgL–1 showed 0.90, 1.00 and 0.99 mm. Regarding the percentage of contamination, 14.33% was obtained. while the percentage of oxidation in the oxyl explants from 25 to 32.66%.

Translated title of the contributionInduction of callogenesis in explants of Vitex mollis Kunth plant genetic resource of the low deciduous forest
Original languageSpanish
Pages (from-to)77-85
Number of pages9
JournalIdesia
Volume41
Issue number2
DOIs
StatePublished - 2023

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