TY - JOUR
T1 - Production of a full chimeric mouse x pig anti-porcine DEC205 receptor recombinant antibody
AU - Bustamante-Córdova, Lorena
AU - Melgoza-González, Edgar Alonso
AU - Parra-Sánchez, Héctor
AU - Reséndiz-Sandoval, Mónica
AU - Burgara-Estrella, Alexel Jesús
AU - Hernández, Jesús
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/2
Y1 - 2021/2
N2 - Recombinant hybrid antibodies are commonly used in antigen-targeting assays to reduce the immunogenic potential associated with using classic mouse antibodies in other species. The DEC205 receptor has become an attractive target due to its effectiveness in activating the immune response and is considered a promising vaccination target. The aim of this study was to produce a fully chimeric mouse x pig anti-porcine DEC205 recombinant antibody (rAb). Based on a mouse anti-porcine DEC205 monoclonal antibody (mAb), we designed and expressed a chimeric mouse x pig rAb using the Expi293f system. The resulting rAb maintained the recognition capacity of the native mouse mAb toward the porcine DEC205 receptor, as evidenced by western blot analysis. By using flow cytometry, we evaluated the ability of the rAb to recognize DEC205+ dendritic cells. In conclusion, the chimeric mouse x pig anti-DEC205 rAb can be used in antigen-targeting assays as a vaccination strategy in pigs.
AB - Recombinant hybrid antibodies are commonly used in antigen-targeting assays to reduce the immunogenic potential associated with using classic mouse antibodies in other species. The DEC205 receptor has become an attractive target due to its effectiveness in activating the immune response and is considered a promising vaccination target. The aim of this study was to produce a fully chimeric mouse x pig anti-porcine DEC205 recombinant antibody (rAb). Based on a mouse anti-porcine DEC205 monoclonal antibody (mAb), we designed and expressed a chimeric mouse x pig rAb using the Expi293f system. The resulting rAb maintained the recognition capacity of the native mouse mAb toward the porcine DEC205 receptor, as evidenced by western blot analysis. By using flow cytometry, we evaluated the ability of the rAb to recognize DEC205+ dendritic cells. In conclusion, the chimeric mouse x pig anti-DEC205 rAb can be used in antigen-targeting assays as a vaccination strategy in pigs.
KW - Antibody production
KW - Chimeric antibody
KW - DEC205
KW - Dendritic cell
KW - Recombinant antibody
KW - Swine
UR - http://www.scopus.com/inward/record.url?scp=85096498824&partnerID=8YFLogxK
U2 - 10.1016/j.jim.2020.112911
DO - 10.1016/j.jim.2020.112911
M3 - Artículo
C2 - 33186587
AN - SCOPUS:85096498824
SN - 0022-1759
VL - 489
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
M1 - 112911
ER -