Progesterone induces NFκB DNA binding activity through a PI3K/Akt-dependent pathway in MCF-7 breast cancer cells

Fernando Candanedo-Gonzalez, Octavio Galindo-Hernandez, Nathalia Serna-Marquez, Roberto Espinosa-Neira, Adriana Soto-Guzman, Pedro Cortes-Reynosa, Eduardo Perez Salazar

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Progesterone (PG) is a steroid hormone that regulates normal reproductive functions including uterine and mammary gland development. The PG receptor belongs to the nuclear receptor superfamily of ligand dependent transcription factors that mediates gene expression, however, it also promotes cell signaling pathways through a non-genomic pathway including the activation of PI3K/Akt and Src/ERK1/2 pathways. However the role of PG in NFκB-DNA complex formation remains to be studied. We demonstrate here that PG induces Akt2 activation and an increase of NFκB DNA binding activity in MCF-7 breast cancer cells. Akt2 activation requires PI3K activity, whereas increase of NFκB DNA binding activity requires PI3K, mTOR, Akt, Src, and G-proteins activity, as well as the integrity of cytoskeleton. In summary, our findings demonstrate that PG induces an increase of NFκB DNA binding activity in MCF-7 cells.
Original languageSpanish (Mexico)
Pages (from-to)63-69
Number of pages7
JournalCancer Research Journal
Volume2
Issue number4
DOIs
StatePublished - 2014

Cite this

Candanedo-Gonzalez, F., Galindo-Hernandez, O., Serna-Marquez, N., Espinosa-Neira, R., Soto-Guzman, A., Cortes-Reynosa, P., & Perez Salazar, E. (2014). Progesterone induces NFκB DNA binding activity through a PI3K/Akt-dependent pathway in MCF-7 breast cancer cells. Cancer Research Journal, 2(4), 63-69. https://doi.org/10.11648/j.crj.20140204.11
Candanedo-Gonzalez, Fernando ; Galindo-Hernandez, Octavio ; Serna-Marquez, Nathalia ; Espinosa-Neira, Roberto ; Soto-Guzman, Adriana ; Cortes-Reynosa, Pedro ; Perez Salazar, Eduardo. / Progesterone induces NFκB DNA binding activity through a PI3K/Akt-dependent pathway in MCF-7 breast cancer cells. In: Cancer Research Journal. 2014 ; Vol. 2, No. 4. pp. 63-69.
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title = "Progesterone induces NFκB DNA binding activity through a PI3K/Akt-dependent pathway in MCF-7 breast cancer cells",
abstract = "Progesterone (PG) is a steroid hormone that regulates normal reproductive functions including uterine and mammary gland development. The PG receptor belongs to the nuclear receptor superfamily of ligand dependent transcription factors that mediates gene expression, however, it also promotes cell signaling pathways through a non-genomic pathway including the activation of PI3K/Akt and Src/ERK1/2 pathways. However the role of PG in NFκB-DNA complex formation remains to be studied. We demonstrate here that PG induces Akt2 activation and an increase of NFκB DNA binding activity in MCF-7 breast cancer cells. Akt2 activation requires PI3K activity, whereas increase of NFκB DNA binding activity requires PI3K, mTOR, Akt, Src, and G-proteins activity, as well as the integrity of cytoskeleton. In summary, our findings demonstrate that PG induces an increase of NFκB DNA binding activity in MCF-7 cells.",
keywords = "Breast Cancer, MCF-7, NFκB, PI3K/Akt, Progesterone",
author = "Fernando Candanedo-Gonzalez and Octavio Galindo-Hernandez and Nathalia Serna-Marquez and Roberto Espinosa-Neira and Adriana Soto-Guzman and Pedro Cortes-Reynosa and {Perez Salazar}, Eduardo",
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language = "Espa{\~n}ol (M{\'e}xico)",
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Candanedo-Gonzalez, F, Galindo-Hernandez, O, Serna-Marquez, N, Espinosa-Neira, R, Soto-Guzman, A, Cortes-Reynosa, P & Perez Salazar, E 2014, 'Progesterone induces NFκB DNA binding activity through a PI3K/Akt-dependent pathway in MCF-7 breast cancer cells', Cancer Research Journal, vol. 2, no. 4, pp. 63-69. https://doi.org/10.11648/j.crj.20140204.11

Progesterone induces NFκB DNA binding activity through a PI3K/Akt-dependent pathway in MCF-7 breast cancer cells. / Candanedo-Gonzalez, Fernando; Galindo-Hernandez, Octavio; Serna-Marquez, Nathalia; Espinosa-Neira, Roberto; Soto-Guzman, Adriana; Cortes-Reynosa, Pedro; Perez Salazar, Eduardo.

In: Cancer Research Journal, Vol. 2, No. 4, 2014, p. 63-69.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Progesterone induces NFκB DNA binding activity through a PI3K/Akt-dependent pathway in MCF-7 breast cancer cells

AU - Candanedo-Gonzalez, Fernando

AU - Galindo-Hernandez, Octavio

AU - Serna-Marquez, Nathalia

AU - Espinosa-Neira, Roberto

AU - Soto-Guzman, Adriana

AU - Cortes-Reynosa, Pedro

AU - Perez Salazar, Eduardo

PY - 2014

Y1 - 2014

N2 - Progesterone (PG) is a steroid hormone that regulates normal reproductive functions including uterine and mammary gland development. The PG receptor belongs to the nuclear receptor superfamily of ligand dependent transcription factors that mediates gene expression, however, it also promotes cell signaling pathways through a non-genomic pathway including the activation of PI3K/Akt and Src/ERK1/2 pathways. However the role of PG in NFκB-DNA complex formation remains to be studied. We demonstrate here that PG induces Akt2 activation and an increase of NFκB DNA binding activity in MCF-7 breast cancer cells. Akt2 activation requires PI3K activity, whereas increase of NFκB DNA binding activity requires PI3K, mTOR, Akt, Src, and G-proteins activity, as well as the integrity of cytoskeleton. In summary, our findings demonstrate that PG induces an increase of NFκB DNA binding activity in MCF-7 cells.

AB - Progesterone (PG) is a steroid hormone that regulates normal reproductive functions including uterine and mammary gland development. The PG receptor belongs to the nuclear receptor superfamily of ligand dependent transcription factors that mediates gene expression, however, it also promotes cell signaling pathways through a non-genomic pathway including the activation of PI3K/Akt and Src/ERK1/2 pathways. However the role of PG in NFκB-DNA complex formation remains to be studied. We demonstrate here that PG induces Akt2 activation and an increase of NFκB DNA binding activity in MCF-7 breast cancer cells. Akt2 activation requires PI3K activity, whereas increase of NFκB DNA binding activity requires PI3K, mTOR, Akt, Src, and G-proteins activity, as well as the integrity of cytoskeleton. In summary, our findings demonstrate that PG induces an increase of NFκB DNA binding activity in MCF-7 cells.

KW - Breast Cancer

KW - MCF-7

KW - NFκB

KW - PI3K/Akt

KW - Progesterone

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U2 - 10.11648/j.crj.20140204.11

DO - 10.11648/j.crj.20140204.11

M3 - Artículo

VL - 2

SP - 63

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JO - Cancer Research Journal

JF - Cancer Research Journal

SN - 2330-8214

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