TY - JOUR
T1 - Properties of Cephalopod Skin Ommochromes to Inhibit Free Radicals, and the Maillard Reaction and Retino-Protective Mechanisms in Cellular Models Concerning Oxidative Stress, Angiogenesis, and Inflammation
AU - Lewis, Luján Lidianys María
AU - Dörschmann, Philipp
AU - Seeba, Charlotte
AU - Thalenhorst, Tabea
AU - Roider, Johann
AU - Iloki Assanga, Simon Bernard
AU - Ruiz, Juan Carlos Gálvez
AU - Del Castillo Castro, Teresa
AU - Rosas-Burgos, Ema Carina
AU - Plascencia-Jatomea, Maribel
AU - Ezquerra Brauer, Josafat Marina
AU - Klettner, Alexa
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/8
Y1 - 2022/8
N2 - Ommochromes are pigments of invertebrates that exhibit oxidative stress protection. The aim of this study was to investigate ommochromes extracted from cephalopod’s skin for their ability to inhibit age-related-macular degeneration (AMD)-related factors such as H2O2-induced and iron-dependent oxidative stress (ferroptosis and erastin), accumulation of advanced glycation end-products (AGEs), as well as vascular endothelial growth factor (VEGF), and inflammatory cytokines (interleukin 6 and interleukin 8) secretion. As cell systems, we used primary porcine retinal pigment epithelium (RPE), human retinal pigment epithelium cell line ARPE-19 and uveal melanoma cell line OMM-1. In vitro, ommochromes produced an antiglycation effect by the inhibition of fructosylation reaction. The ommochromes showed protective effects against erastin- induced cell death in ARPE-19. In addition, in long-term stimulation (7 days) ommochromes decreased constitutively secreted VEGF, as well as interleukin 6 and interleukin 8 induced by Poly I:C in primary RPE. No relevant effects were detected in OMM-1 cells. The effects are dependent on the cell system, time of exposition, and concentration. This substance is of interest for further research concerning age-related macular degeneration.
AB - Ommochromes are pigments of invertebrates that exhibit oxidative stress protection. The aim of this study was to investigate ommochromes extracted from cephalopod’s skin for their ability to inhibit age-related-macular degeneration (AMD)-related factors such as H2O2-induced and iron-dependent oxidative stress (ferroptosis and erastin), accumulation of advanced glycation end-products (AGEs), as well as vascular endothelial growth factor (VEGF), and inflammatory cytokines (interleukin 6 and interleukin 8) secretion. As cell systems, we used primary porcine retinal pigment epithelium (RPE), human retinal pigment epithelium cell line ARPE-19 and uveal melanoma cell line OMM-1. In vitro, ommochromes produced an antiglycation effect by the inhibition of fructosylation reaction. The ommochromes showed protective effects against erastin- induced cell death in ARPE-19. In addition, in long-term stimulation (7 days) ommochromes decreased constitutively secreted VEGF, as well as interleukin 6 and interleukin 8 induced by Poly I:C in primary RPE. No relevant effects were detected in OMM-1 cells. The effects are dependent on the cell system, time of exposition, and concentration. This substance is of interest for further research concerning age-related macular degeneration.
KW - cephalopods
KW - ferroptosis
KW - glycative stress
KW - interleukin
KW - ommochromes
KW - retinal pigment epithelium
KW - uveal melanoma
KW - vascular endothelial growth factor
UR - http://www.scopus.com/inward/record.url?scp=85137347649&partnerID=8YFLogxK
U2 - 10.3390/antiox11081574
DO - 10.3390/antiox11081574
M3 - Artículo
C2 - 36009293
AN - SCOPUS:85137347649
SN - 2076-3921
VL - 11
JO - Antioxidants
JF - Antioxidants
IS - 8
M1 - 1574
ER -