Properties of Cephalopod Skin Ommochromes to Inhibit Free Radicals, and the Maillard Reaction and Retino-Protective Mechanisms in Cellular Models Concerning Oxidative Stress, Angiogenesis, and Inflammation

Luján Lidianys María Lewis, Philipp Dörschmann, Charlotte Seeba, Tabea Thalenhorst, Johann Roider, Simon Bernard Iloki Assanga, Juan Carlos Gálvez Ruiz, Teresa Del Castillo Castro, Ema Carina Rosas-Burgos, Maribel Plascencia-Jatomea, Josafat Marina Ezquerra Brauer, Alexa Klettner*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Ommochromes are pigments of invertebrates that exhibit oxidative stress protection. The aim of this study was to investigate ommochromes extracted from cephalopod’s skin for their ability to inhibit age-related-macular degeneration (AMD)-related factors such as H2O2-induced and iron-dependent oxidative stress (ferroptosis and erastin), accumulation of advanced glycation end-products (AGEs), as well as vascular endothelial growth factor (VEGF), and inflammatory cytokines (interleukin 6 and interleukin 8) secretion. As cell systems, we used primary porcine retinal pigment epithelium (RPE), human retinal pigment epithelium cell line ARPE-19 and uveal melanoma cell line OMM-1. In vitro, ommochromes produced an antiglycation effect by the inhibition of fructosylation reaction. The ommochromes showed protective effects against erastin- induced cell death in ARPE-19. In addition, in long-term stimulation (7 days) ommochromes decreased constitutively secreted VEGF, as well as interleukin 6 and interleukin 8 induced by Poly I:C in primary RPE. No relevant effects were detected in OMM-1 cells. The effects are dependent on the cell system, time of exposition, and concentration. This substance is of interest for further research concerning age-related macular degeneration.

Original languageEnglish
Article number1574
JournalAntioxidants
Volume11
Issue number8
DOIs
StatePublished - Aug 2022
Externally publishedYes

Bibliographical note

Funding Information:
L.M.L.L. acknowledges Alexander Dontsov and Marina Yakovleva for the generous scientific and technical support in the antioxidant, antiglycation activity, and HPLC experiments. Additionally, L.M.L.L would like to thank Leila Deravi (Northeastern University, Boston, Massachusetts for their technical help in FTIR and NMR spectrums and the partial financing by project number 2174 (agreement: I0000/230/2018) of the National Council of Science and Technology (CONACYT) of Mexico and for the doctoral fellowship. A.K. and P.D. thank the DFG (German Research Foundation) for their support in initiating this cooperation (grant number KL 2425/13-1).

Publisher Copyright:
© 2022 by the authors.

Keywords

  • cephalopods
  • ferroptosis
  • glycative stress
  • interleukin
  • ommochromes
  • retinal pigment epithelium
  • uveal melanoma
  • vascular endothelial growth factor

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