Proposal of a five MIRU-VNTR panel to screen clinical isolates of Mycobacterium tuberculosis in Mexico

Enrique Bolado-Martínez, Maria Del Carmen Candia-Plata, Roberto Zenteno-Cuevas, Fabiola Mendoza Damián, Magali Avilés-Acosta, Gerardo Álvarez-Hernández

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

© 2014 Elsevier España, S.L.U. y Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. Introduction Tuberculosis is a public health problem across Mexico. This paper aims to select a panel, with a minimum number of repetitive elements (MIRU-VNTR) for genotypic characterization of Mycobacterium tuberculosis (M. tuberculosis) clinical isolates. Method In this study, a full panel of 24 MIRU-VNTR loci was used to discriminate 65 clinical isolates of M. tuberculosis from three different geographical regions of Mexico. Those loci with the highest discriminatory power were subsequently selected. Results The panel, including five loci, was obtained by selecting the highest values of allelic diversity among the genotypes obtained. The dendrogram, generated by the panel MIRU-VNTR 5, showed a high discriminatory power with 65 unique genotype profiles and formed clusters according to the geographical region of origin. Conclusions The panel MIRU-VNTR 5 can be useful for characterizing clinical isolates of M. tuberculosis in Mexico.
Original languageAmerican English
Pages (from-to)609-612
Number of pages4
JournalEnfermedades Infecciosas y Microbiologia Clinica
DOIs
StatePublished - 1 Jan 2015

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