Protease activity and partial characterization of the trypsin-like enzyme in the digestive tract of the tropical sierra Scomberomorus concolor

Hermenegildo Olivas-Burrola; Josafat Marina Ezquerra-Brauer; Ofelia Rouzaud-Sandez; Ramon Pacheco-Aguilar

doi:10.1300/J030v10n04_05

Protease activity and partial characterization of the trypsin-like enzyme in the digestive tract of the tropical sierra Scomberomorus concolor

Hermenegildo Olivas-Burrola
, Josafat Marina Ezquerra-Brauer^*
, Ofelia Rouzaud-Sandez
, Ramon Pacheco-Aguilar

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Protease and trypsin-like activities of the digestive system of the tropical sierra Scomberomorus concolor were evaluated. Sierra digestive tract extracts hydrolyzed specific substrates for trypsin, chymotrypsin, and leucine aminopeptidase. At least eight bands of activity were observed by using SDS-PAGE. In PAGE and serine-inhibition assays, one fraction resembled bovine trypsin. An ammonium sulfate (40-60%) trypsin-like fraction displayed different inhibitions with tosyllys-chloromethyl ketone (96%) and soybean trypsin inhibitor (100%). Maximum activity was found using benzoyl-L-arginine-p-nitroanilide as substrate at pH 9.0 at 25°C and around 50°C at pH 7.8. The capacity of the trypsin-like fraction to work at different pHs (4, 7, and 9) at 25°C and different temperatures (0 and 40°C) at pH 7.8 were detected.

Original language	English
Pages (from-to)	51-64
Number of pages	14
Journal	Journal of Aquatic Food Product Technology
Volume	10
Issue number	4
DOIs	https://doi.org/10.1300/J030v10n04_05
State	Published - 1 Dec 2001
Externally published	Yes

Keywords

Digestive tract
Protease activity
Scomberomorus concolor
Tropical fish

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10.1300/J030v10n04_05

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title = "Protease activity and partial characterization of the trypsin-like enzyme in the digestive tract of the tropical sierra Scomberomorus concolor",

abstract = "Protease and trypsin-like activities of the digestive system of the tropical sierra Scomberomorus concolor were evaluated. Sierra digestive tract extracts hydrolyzed specific substrates for trypsin, chymotrypsin, and leucine aminopeptidase. At least eight bands of activity were observed by using SDS-PAGE. In PAGE and serine-inhibition assays, one fraction resembled bovine trypsin. An ammonium sulfate (40-60\%) trypsin-like fraction displayed different inhibitions with tosyllys-chloromethyl ketone (96\%) and soybean trypsin inhibitor (100\%). Maximum activity was found using benzoyl-L-arginine-p-nitroanilide as substrate at pH 9.0 at 25°C and around 50°C at pH 7.8. The capacity of the trypsin-like fraction to work at different pHs (4, 7, and 9) at 25°C and different temperatures (0 and 40°C) at pH 7.8 were detected.",

keywords = "Digestive tract, Protease activity, Scomberomorus concolor, Tropical fish",

author = "Hermenegildo Olivas-Burrola and Ezquerra-Brauer, \{Josafat Marina\} and Ofelia Rouzaud-Sandez and Ramon Pacheco-Aguilar",

year = "2001",

month = dec,

day = "1",

doi = "10.1300/J030v10n04\_05",

language = "Ingl{\'e}s",

volume = "10",

pages = "51--64",

journal = "Journal of Aquatic Food Product Technology",

issn = "1049-8850",

publisher = "Taylor and Francis Ltd.",

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}

Protease activity and partial characterization of the trypsin-like enzyme in the digestive tract of the tropical sierra Scomberomorus concolor. / Olivas-Burrola, Hermenegildo; Ezquerra-Brauer, Josafat Marina ; Rouzaud-Sandez, Ofelia et al.
In: Journal of Aquatic Food Product Technology, Vol. 10, No. 4, 01.12.2001, p. 51-64.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Protease activity and partial characterization of the trypsin-like enzyme in the digestive tract of the tropical sierra Scomberomorus concolor

AU - Olivas-Burrola, Hermenegildo

AU - Ezquerra-Brauer, Josafat Marina

AU - Rouzaud-Sandez, Ofelia

AU - Pacheco-Aguilar, Ramon

PY - 2001/12/1

Y1 - 2001/12/1

N2 - Protease and trypsin-like activities of the digestive system of the tropical sierra Scomberomorus concolor were evaluated. Sierra digestive tract extracts hydrolyzed specific substrates for trypsin, chymotrypsin, and leucine aminopeptidase. At least eight bands of activity were observed by using SDS-PAGE. In PAGE and serine-inhibition assays, one fraction resembled bovine trypsin. An ammonium sulfate (40-60%) trypsin-like fraction displayed different inhibitions with tosyllys-chloromethyl ketone (96%) and soybean trypsin inhibitor (100%). Maximum activity was found using benzoyl-L-arginine-p-nitroanilide as substrate at pH 9.0 at 25°C and around 50°C at pH 7.8. The capacity of the trypsin-like fraction to work at different pHs (4, 7, and 9) at 25°C and different temperatures (0 and 40°C) at pH 7.8 were detected.

AB - Protease and trypsin-like activities of the digestive system of the tropical sierra Scomberomorus concolor were evaluated. Sierra digestive tract extracts hydrolyzed specific substrates for trypsin, chymotrypsin, and leucine aminopeptidase. At least eight bands of activity were observed by using SDS-PAGE. In PAGE and serine-inhibition assays, one fraction resembled bovine trypsin. An ammonium sulfate (40-60%) trypsin-like fraction displayed different inhibitions with tosyllys-chloromethyl ketone (96%) and soybean trypsin inhibitor (100%). Maximum activity was found using benzoyl-L-arginine-p-nitroanilide as substrate at pH 9.0 at 25°C and around 50°C at pH 7.8. The capacity of the trypsin-like fraction to work at different pHs (4, 7, and 9) at 25°C and different temperatures (0 and 40°C) at pH 7.8 were detected.

KW - Digestive tract

KW - Protease activity

KW - Scomberomorus concolor

KW - Tropical fish

UR - https://www.scopus.com/pages/publications/0035705123

U2 - 10.1300/J030v10n04_05

DO - 10.1300/J030v10n04_05

M3 - Artículo

SN - 1049-8850

VL - 10

SP - 51

EP - 64

JO - Journal of Aquatic Food Product Technology

JF - Journal of Aquatic Food Product Technology

IS - 4

ER -

Protease activity and partial characterization of the trypsin-like enzyme in the digestive tract of the tropical sierra Scomberomorus concolor

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Keywords

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