Protective effect of Caffeic Acid Phenethyl Ester (CAPE) against oxidative stress

Ana Laura Carreño, Efrain Alday, Jael Quintero, Lucía Pérez, Dora Valencia, Ramón Robles-Zepeda, Judith Valdez-Ortega, Javier Hernandez, Carlos Velazquez*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


The antioxidant properties of several polyphenolics of propolis have been reported, however their protective effect against oxidative stress considering cell integrity is scarce. In this study, we evaluated the cellular antioxidant activity (CAA) of caffeic acid phenetyl ester (CAPE), rutin and galangin (Sonoran propolis constituents) using two murine cell lines derived from different immunological lineages (B-cell lymphoma and macrophages), based on the fluorescence of intracellularly oxidised 2′-7′-dichlorofluorescein (DCF) probe, together with cell morphology analysis and membrane integrity assessment by flow cytometry. CAPE (5 μM) showed the highest CAA (97.9%) on B-cell lymphoma cells against 1 mM H2O2, followed by rutin (25 μM; 30.9%), meanwhile galangin (25 μM) did not show CAA. CAPE exhibited a higher CAA than the antioxidant controls [quercetin (12.5 μM), ascorbic acid (50 μM) and trolox (10 μM)], and additionally it helped to preserve cellular morphology. Similar effects were observed on macrophage cells, indicating that CAPE has a cellular protective effect against ROS. Further studies are needed to investigate the potential health benefits of CAPE.

Original languageEnglish
Pages (from-to)178-184
Number of pages7
JournalJournal of Functional Foods
StatePublished - 1 Feb 2017

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd


  • CAPE
  • Cellular antioxidant activity
  • Murine B-cell lymphoma cells
  • Murine macrophage cells
  • Sonoran propolis constituents


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