Recombinant bacterial expression of the lysozyme from the tobacco-hornworm Manduca sexta with activity at low temperatures

Karina D. García-Orozco; Alonso A. López-Zavala; Daniel Puentes-Camacho; Ana Maria Calderón-De-La-Barca; Rogerio R. Sotelo-Mundo

doi:10.1007/s10529-005-8452-1

Recombinant bacterial expression of the lysozyme from the tobacco-hornworm Manduca sexta with activity at low temperatures

Karina D. García-Orozco, Alonso A. López-Zavala, Daniel Puentes-Camacho, Ana Maria Calderón-De-La-Barca, Rogerio R. Sotelo-Mundo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A gene coding for lysozyme from the insect Manduca sexta (Ms-lyz) was expressed in Escherichia coli. The protein was produced as an insoluble cytoplasmic inclusion body which was denatured in 8 M guanidine-HCl, renatured and purified by affinity and ion-exchange chromatography. The N-terminal sequence and the activity of the recombinant protein against Micrococcus luteus confirmed that correct expression had occurred. When Ms-lyz activity was compared to hen egg white lysozyme, the insect lysozyme was active at lower temperatures. These results demonstrate the feasibility of producing a disulfide-bonded lysozyme enzyme in bacteria and suggest that the insect Ms-lyz is an interesting system for further development of an antibacterial functional at low temperatures.

Original language	English
Pages (from-to)	1075-1080
Number of pages	6
Journal	Biotechnology Letters
Volume	27
Issue number	15
DOIs	https://doi.org/10.1007/s10529-005-8452-1
State	Published - Aug 2005
Externally published	Yes

Bibliographical note

Funding Information:
This work was financed by grants 36928-B and 41184-Z from CONACyT (National Research Council of Science and Technology of México) and by grant A/3230-1 from International Foundation for Science (Sweden). A. López-Zavala and D. Puentes-Camacho received scholarships from CONACyT. We thank Drs. Maria A. Islas-Osuna and Mayra de la Torre from CIAD (Sonora, México) and Dr. Lorraine Marnell from University of New Mexico (NM, USA) for critical reading and editorial suggestions during the preparation of this manuscript.

Keywords

Affinity chromatography
Bacterial over-expression
Lysozyme
Manduca sexta
Refolding

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10.1007/s10529-005-8452-1

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title = "Recombinant bacterial expression of the lysozyme from the tobacco-hornworm Manduca sexta with activity at low temperatures",

abstract = "A gene coding for lysozyme from the insect Manduca sexta (Ms-lyz) was expressed in Escherichia coli. The protein was produced as an insoluble cytoplasmic inclusion body which was denatured in 8 M guanidine-HCl, renatured and purified by affinity and ion-exchange chromatography. The N-terminal sequence and the activity of the recombinant protein against Micrococcus luteus confirmed that correct expression had occurred. When Ms-lyz activity was compared to hen egg white lysozyme, the insect lysozyme was active at lower temperatures. These results demonstrate the feasibility of producing a disulfide-bonded lysozyme enzyme in bacteria and suggest that the insect Ms-lyz is an interesting system for further development of an antibacterial functional at low temperatures.",

keywords = "Affinity chromatography, Bacterial over-expression, Lysozyme, Manduca sexta, Refolding",

author = "Garc{\'i}a-Orozco, {Karina D.} and L{\'o}pez-Zavala, {Alonso A.} and Daniel Puentes-Camacho and Calder{\'o}n-De-La-Barca, {Ana Maria} and Sotelo-Mundo, {Rogerio R.}",

note = "Funding Information: This work was financed by grants 36928-B and 41184-Z from CONACyT (National Research Council of Science and Technology of M{\'e}xico) and by grant A/3230-1 from International Foundation for Science (Sweden). A. L{\'o}pez-Zavala and D. Puentes-Camacho received scholarships from CONACyT. We thank Drs. Maria A. Islas-Osuna and Mayra de la Torre from CIAD (Sonora, M{\'e}xico) and Dr. Lorraine Marnell from University of New Mexico (NM, USA) for critical reading and editorial suggestions during the preparation of this manuscript.",

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doi = "10.1007/s10529-005-8452-1",

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AU - García-Orozco, Karina D.

AU - López-Zavala, Alonso A.

AU - Puentes-Camacho, Daniel

AU - Calderón-De-La-Barca, Ana Maria

AU - Sotelo-Mundo, Rogerio R.

N1 - Funding Information: This work was financed by grants 36928-B and 41184-Z from CONACyT (National Research Council of Science and Technology of México) and by grant A/3230-1 from International Foundation for Science (Sweden). A. López-Zavala and D. Puentes-Camacho received scholarships from CONACyT. We thank Drs. Maria A. Islas-Osuna and Mayra de la Torre from CIAD (Sonora, México) and Dr. Lorraine Marnell from University of New Mexico (NM, USA) for critical reading and editorial suggestions during the preparation of this manuscript.

PY - 2005/8

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N2 - A gene coding for lysozyme from the insect Manduca sexta (Ms-lyz) was expressed in Escherichia coli. The protein was produced as an insoluble cytoplasmic inclusion body which was denatured in 8 M guanidine-HCl, renatured and purified by affinity and ion-exchange chromatography. The N-terminal sequence and the activity of the recombinant protein against Micrococcus luteus confirmed that correct expression had occurred. When Ms-lyz activity was compared to hen egg white lysozyme, the insect lysozyme was active at lower temperatures. These results demonstrate the feasibility of producing a disulfide-bonded lysozyme enzyme in bacteria and suggest that the insect Ms-lyz is an interesting system for further development of an antibacterial functional at low temperatures.

AB - A gene coding for lysozyme from the insect Manduca sexta (Ms-lyz) was expressed in Escherichia coli. The protein was produced as an insoluble cytoplasmic inclusion body which was denatured in 8 M guanidine-HCl, renatured and purified by affinity and ion-exchange chromatography. The N-terminal sequence and the activity of the recombinant protein against Micrococcus luteus confirmed that correct expression had occurred. When Ms-lyz activity was compared to hen egg white lysozyme, the insect lysozyme was active at lower temperatures. These results demonstrate the feasibility of producing a disulfide-bonded lysozyme enzyme in bacteria and suggest that the insect Ms-lyz is an interesting system for further development of an antibacterial functional at low temperatures.

KW - Affinity chromatography

KW - Bacterial over-expression

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KW - Manduca sexta

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SP - 1075

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JO - Biotechnology Letters

JF - Biotechnology Letters

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ER -

Recombinant bacterial expression of the lysozyme from the tobacco-hornworm Manduca sexta with activity at low temperatures

Abstract

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Keywords

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