TY - JOUR
T1 - Regulation of lactate dehydrogenase in response to WSSV infection in the shrimp Litopenaeus vannamei
AU - Hernández-Palomares, M. L.E.
AU - Godoy-Lugo, J. A.
AU - Gómez-Jiménez, S.
AU - Gámez-Alejo, L. A.
AU - Ortiz, R. M.
AU - Muñoz-Valle, J. F.
AU - Peregrino-Uriarte, A. B.
AU - Yepiz-Plascencia, G.
AU - Rosas-Rodríguez, J. A.
AU - Soñanez-Organis, J. G.
N1 - Publisher Copyright:
© 2018
PY - 2018/3/1
Y1 - 2018/3/1
N2 - Lactate dehydrogenase (LDH) is key for anaerobic glycolysis. LDH is induced by the hypoxia inducible factor −1 (HIF-1). HIF-1 induces genes involved in glucose metabolism and regulates cellular oxygen homeostasis. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces anaerobic glycolysis in shrimp hemocytes, associated with lactate accumulation. Although infection and lactate production are associated, the LDH role in WSSV-infected shrimp has not been examined. In this work, the effects of HIF-1 silencing on the expression of two LDH subunits (LDHvan-1 and LDHvan-2) in shrimp infected with the WSSV were studied. HIF-1α transcripts increased in gills, hepatopancreas, and muscle after WSSV infection, while HIF-1β remained constitutively expressed. The expression for both LDH subunits increased in each tissue evaluated during the WSSV infection, translating into increased enzyme activity. Glucose concentration increased in each tissue evaluated, while lactate increased in gills and hepatopancreas, but not in muscle. Silencing of HIF-1α blocked the increase of LDH expression and enzyme activity, along with glucose (all tissues) and lactate (gills and hepatopancreas) concentrations produced by WSSV infection. These results demonstrate that HIF-1 up regulates the expression of LDH subunits during WSSV infection, and that this induction contributes to substrate metabolism in energetically active tissues of infected shrimp.
AB - Lactate dehydrogenase (LDH) is key for anaerobic glycolysis. LDH is induced by the hypoxia inducible factor −1 (HIF-1). HIF-1 induces genes involved in glucose metabolism and regulates cellular oxygen homeostasis. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces anaerobic glycolysis in shrimp hemocytes, associated with lactate accumulation. Although infection and lactate production are associated, the LDH role in WSSV-infected shrimp has not been examined. In this work, the effects of HIF-1 silencing on the expression of two LDH subunits (LDHvan-1 and LDHvan-2) in shrimp infected with the WSSV were studied. HIF-1α transcripts increased in gills, hepatopancreas, and muscle after WSSV infection, while HIF-1β remained constitutively expressed. The expression for both LDH subunits increased in each tissue evaluated during the WSSV infection, translating into increased enzyme activity. Glucose concentration increased in each tissue evaluated, while lactate increased in gills and hepatopancreas, but not in muscle. Silencing of HIF-1α blocked the increase of LDH expression and enzyme activity, along with glucose (all tissues) and lactate (gills and hepatopancreas) concentrations produced by WSSV infection. These results demonstrate that HIF-1 up regulates the expression of LDH subunits during WSSV infection, and that this induction contributes to substrate metabolism in energetically active tissues of infected shrimp.
KW - Gene expression
KW - Gene silencing
KW - Hypoxia-inducible factor -1α
KW - Lactate dehydrogenase
KW - White spot syndrome virus
UR - http://www.scopus.com/inward/record.url?scp=85043315701&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2018.01.011
DO - 10.1016/j.fsi.2018.01.011
M3 - Artículo
C2 - 29337249
SN - 1050-4648
VL - 74
SP - 401
EP - 409
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
ER -